HUMAN PLASMA GELSOLIN BINDS ADENOSINE-TRIPHOSPHATE

被引：12

作者：

YAMAMOTO, H ^{[1
]}

ITO, H ^{[1
]}

NAKAMURA, H ^{[1
]}

HAYASHI, E ^{[1
]}

KISHIMOTO, S ^{[1
]}

HASHIMOTO, T ^{[1
]}

TAGAWA, K ^{[1
]}

机构：

[1] OSAKA UNIV,SCH MED,DEPT PHYSIOL CHEM,KITA KU,OSAKA 530,JAPAN

来源：

JOURNAL OF BIOCHEMISTRY | 1990年 / 108卷 / 04期

关键词：

D O I：

10.1093/oxfordjournals.jbchem.a123229

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Binding studies of human plasma gelsolin with ATP were done by equilibrium dialysis. Analysis of the binding data showed that plasma gelsolin had one class of ATP binding site with Kd = 2.8 × 10-7 M, which saturated at an ATP/gelsolin ratio of 0.6. The bioluminescent assay for ATP with luciferin and firefly luciferase confirmed that the protein contained a nucleotide as ATP. © 1990 Copyright, 1990 by the Journal of Biochemistry.

引用

页码：505 / 506

页数：2