HUMAN PLASMA GELSOLIN BINDS ADENOSINE-TRIPHOSPHATE

被引：12

作者：

YAMAMOTO, H ^{[1
]}

ITO, H ^{[1
]}

NAKAMURA, H ^{[1
]}

HAYASHI, E ^{[1
]}

KISHIMOTO, S ^{[1
]}

HASHIMOTO, T ^{[1
]}

TAGAWA, K ^{[1
]}

机构：

[1] OSAKA UNIV,SCH MED,DEPT PHYSIOL CHEM,KITA KU,OSAKA 530,JAPAN

来源：

JOURNAL OF BIOCHEMISTRY | 1990年 / 108卷 / 04期

关键词：

D O I：

10.1093/oxfordjournals.jbchem.a123229

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Binding studies of human plasma gelsolin with ATP were done by equilibrium dialysis. Analysis of the binding data showed that plasma gelsolin had one class of ATP binding site with Kd = 2.8 × 10-7 M, which saturated at an ATP/gelsolin ratio of 0.6. The bioluminescent assay for ATP with luciferin and firefly luciferase confirmed that the protein contained a nucleotide as ATP. © 1990 Copyright, 1990 by the Journal of Biochemistry.

引用

页码：505 / 506

页数：2

共 21 条

[1] DEFINITION OF AN N-TERMINAL ACTIN-BINDING DOMAIN AND A C-TERMINAL CA-2+ REGULATORY DOMAIN IN HUMAN BREVIN
BRYAN, J
HWO, S
[J]. JOURNAL OF CELL BIOLOGY, 1986, 102 (04) : 1439 - 1446
[2] GELSOLIN HAS 3 ACTIN-BINDING SITES
BRYAN, J
[J]. JOURNAL OF CELL BIOLOGY, 1988, 106 (05) : 1553 - 1562
[3] COUE M, 1986, J BIOL CHEM, V261, P1588
[4] LACK OF NUCLEOTIDE CLEAVAGE ON THE BINDING OF G-ACTIN-ATP TO PLASMA GELSOLIN
HARRIS, HE
[J]. FEBS LETTERS, 1985, 190 (01) : 81 - 83
[5] JABS CM, 1977, CLIN CHEM, V23, P2254
[6] JANMEY PA, 1987, J BIOL CHEM, V262, P12228
[7] JANMEY PA, 1989, J BIOL CHEM, V264, P4825
[8] MODULATION OF GELSOLIN FUNCTION BY PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE
JANMEY, PA
STOSSEL, TP
[J]. NATURE, 1987, 325 (6102) : 362 - 364
[9] CLINICAL AND BIOCHEMICAL APPLICATIONS OF LUCIFERASES AND LUCIFERINS
KRICKA, LJ
[J]. ANALYTICAL BIOCHEMISTRY, 1988, 175 (01) : 14 - 21
[10] F-ACTIN-DEPOLYMERIZING ACTIVITY OF HUMAN-SERUM
NORBERG, R
THORSTENSSON, R
UTTER, G
FAGRAEUS, A
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1979, 100 (02): : 575 - 583

← 1 2 3 →