DETECTION OF ESCHERICHIA-COLI O157/H7 BY MULTIPLEX PCR

被引：176

作者：

FRATAMICO, PM ^{[1
]}

SACKITEY, SK ^{[1
]}

WIEDMANN, M ^{[1
]}

DENG, MY ^{[1
]}

机构：

[1] CORNELL UNIV,DEPT FOOD SCI,ITHACA,NY 14853

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1995年 / 33卷 / 08期

关键词：

D O I：

10.1128/JCM.33.8.2188-2191.1995

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

In order to develop a PCR assay for Escherichia coli O157:H7, a portion of the 60-MDa plasmid harbored by enterohemorrhagic E. coli (EHEC) was sequenced and PCR primers were designed. A multiplex PCR method was then designed by employing primers specific for the EHEC eaeA gene, conserved sequences of Shiga-like toxins I (SLT-I) and LI (SLT-II), and the 60-MDa plasmid. PCR products of 1,087 bp (eaeA), 227 and/or 224 bp (SLT-I and/or SLT-II), and 166 bp (plasmid) were successfully amplified simultaneously in a single reaction. The multiplex PCR method can be used to specifically identify EHEC of serogroup O157.

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页码：2188 / 2191

页数：4

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