FTSH, A MEMBRANE-BOUND ATPASE, FORMS A COMPLEX IN THE CYTOPLASMIC MEMBRANE OF ESCHERICHIA-COLI

被引:95
作者
AKIYAMA, Y
YOSHIHISA, T
ITO, K
机构
[1] Department of Cell Biology, Institute for Virus Research, Kyoto University
关键词
D O I
10.1074/jbc.270.40.23485
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The FtsH (HflB) protein of Escherichia coli is inte grated into the membrane with two N-terminally located transmembrane segments, while its large cytoplasmic domain is homologous to the AAA family of ATPases, The previous studies on dominant negative ftsH mutants raised a possibility that FtsH functions in multimeric states, We found that FtsH was eluted at fractions corresponding to a larger molecular weight than expected from monomeric structure in size-exclusion chromatography, Moreover, treatment of membranes or their detergent extracts with a cross-linker, dithiobis(succinimidyl propionate), yielded cross linked products of FtsH, To dissect possible FtsH complex, we constructed an FtsH derivative with c-Myc epitope at its C terminus (FtsH-His(6)-Myc). When membranes prepared from cells in which FtsH-His(6)-Myc was overproduced together with the normal FtsH were treated with the cross-linker, intact FtsH and in vitro degradation products of FtsH-His(6)-Myc without the tag were cross linked with the tagged FtsH protein, Co-immunoprecipitation experiments confirmed the interaction between the FtsH molecules, To identify regions of FtsH required or sufficient for this interaction, we constructed chimeric proteins between FtsH and EnvZ, a protein with a similar topological arrangement, by exchanging their corresponding domains, We found that only the FtsH-EnvZ hybrid protein with an FtsH-derived membrane anchoring domain and an EnvZ-derived cytoplasmic domain caused a dominant ftsH phenotype and was cross-linked with FtsH, We suggest that the N-terminal transmembrane region of FtsH mediates directly the interaction between the FtsH subunits.
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页码:23485 / 23490
页数:6
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