INTERNAL STANDARDIZATION TECHNIQUE FOR CAPILLARY ZONE ELECTROPHORESIS

被引：120

作者：

DOSE, EV

GUIOCHON, GA

机构：

[1] UNIV TENNESSEE,DEPT CHEM,KNOXVILLE,TN 37996

[2] OAK RIDGE NATL LAB,DIV ANALYT CHEM,OAK RIDGE,TN 37831

来源：

ANALYTICAL CHEMISTRY | 1991年 / 63卷 / 11期

关键词：

D O I：

10.1021/ac00011a018

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new method of internal standardization for capillary zone electrophoresis (CZE) is founded on the linear relation between each ion's effective volume injected and its inherent mobility. The use of two internal standards allows the analyst to establish this relation quantitatively for each sample and to correct the analyte concentrations for variations in each injection. The computational method given is simple, and we demonstrate that it can give reproducibilities for manual CZE hydrodynamic and electrostatic injections of under 1% relative standard deviation.

引用

收藏

页码：1154 / 1158

页数：5

相关论文

共 21 条

[1] SUBATTOMOLE AMINO-ACID ANALYSIS BY CAPILLARY ZONE ELECTROPHORESIS AND LASER-INDUCED FLUORESCENCE [J].

CHENG, YF ;

DOVICHI, NJ .

SCIENCE, 1988, 242 (4878) :562-564

[2] HIGH-SPEED SEPARATIONS OF DNA SEQUENCING REACTIONS BY CAPILLARY ELECTROPHORESIS [J].

DROSSMAN, H ;

LUCKEY, JA ;

KOSTICHKA, AJ ;

DCUNHA, J ;

SMITH, LM .

ANALYTICAL CHEMISTRY, 1990, 62 (09) :900-903

[3] DETERMINATION OF INGREDIENTS OF ANTIPYRETIC ANALGESIC PREPARATIONS BY MICELLAR ELECTROKINETIC CAPILLARY CHROMATOGRAPHY [J].

FUJIWARA, S ;

HONDA, S .

ANALYTICAL CHEMISTRY, 1987, 59 (23) :2773-2776

[4] DETERMINATION OF CINNAMIC ACID AND ITS ANALOGS BY ELECTROPHORESIS IN A FUSED-SILICA CAPILLARY-TUBE [J].

FUJIWARA, S ;

HONDA, S .

ANALYTICAL CHEMISTRY, 1986, 58 (08) :1811-1814

[5] CAPILLARY ELECTROPHORESIS [J].

GORDON, MJ ;

HUANG, XH ;

PENTONEY, SL ;

ZARE, RN .

SCIENCE, 1988, 242 (4876) :224-228

[6] INDIRECT FLUORIMETRIC DETECTION AND QUANTIFICATION IN CAPILLARY ZONE ELECTROPHORESIS OF INORGANIC ANIONS AND NUCLEOTIDES [J].

GROSS, L ;

YEUNG, ES .

JOURNAL OF CHROMATOGRAPHY, 1989, 480 :169-178

[7]

GRUSHKA E, 1989, J CHROMATOGR, V471, P431

[8] EVALUATION OF AN AUTOMATIC SIPHONIC SAMPLER FOR CAPILLARY ZONE ELECTROPHORESIS [J].

HONDA, S ;

IWASE, S ;

FUJIWARA, S .

JOURNAL OF CHROMATOGRAPHY, 1987, 404 (02) :313-320

[9] BIAS IN QUANTITATIVE CAPILLARY ZONE ELECTROPHORESIS CAUSED BY ELECTROKINETIC SAMPLE INJECTION [J].

HUANG, XH ;

GORDON, MJ ;

ZARE, RN .

ANALYTICAL CHEMISTRY, 1988, 60 (04) :375-377

[10] QUANTITATIVE-ANALYSIS OF LOW-MOLECULAR WEIGHT CARBOXYLIC-ACIDS BY CAPILLARY ZONE ELECTROPHORESIS CONDUCTIVITY DETECTION [J].

HUANG, XH ;

LUCKEY, JA ;

GORDON, MJ ;

ZARE, RN .

ANALYTICAL CHEMISTRY, 1989, 61 (07) :766-770