PREPARATION OF RABBIT POLYCLONAL ANTIBODIES AGAINST LUPIN STORAGE PROTEINS

被引：4

作者：

BUSH, RS ^{[1
]}

TAI, H ^{[1
]}

机构：

[1] AGR CANADA,RES STN,FREDERICTON E3B 4Z7,NB,CANADA

来源：

CANADIAN JOURNAL OF PLANT SCIENCE | 1994年 / 74卷 / 01期

关键词：

LUPIN; CONGLUTIN; POLYCLONAL ANTIBODY;

D O I：

10.4141/cjps94-016

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Agarose gel electrophoresis was used to separate three globular storage proteins from crude extracts of Lupinus albus seeds. Conglutin alpha, beta and gamma purified by this technique were used as antigens to stimulate the production of antibodies in rabbits. The specificity of polyclonal antibodies produced was analysed by Western blot. Each antibody reacted with the specific antigen used to stimulate production of that antibody. In addition the anti-conglutin alpha preparation also showed binding to a region of the gel that comigrates with conglutin beta.

引用

页码：93 / 96

页数：4

共 13 条

[1] STRUCTURAL SIMILARITY BETWEEN LEGUMIN AND VICILIN STORAGE PROTEINS FROM LEGUMES [J].