ISOLATION OF ELECTRIC PULSE-INDUCED ESCHERICHIA-COLI GENES BY OPERON FUSION TECHNIQUES

被引：0
|
作者：
TSUCHIYA, K [1 ]
MIYAGI, W [1 ]
ANO, T [1 ]
SHODA, M [1 ]
机构：
[1] TOKYO INST TECHNOL,RESOURCES UTILIZAT RES LAB,4259 NAGATSUTA,MIDORI KU,YOKOHAMA,KANAGAWA 227,JAPAN
来源：
BIOTECHNOLOGY TECHNIQUES | 1993年 / 7卷 / 10期
关键词：
D O I：
10.1007/BF00152619
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A search of electric-pulse induced genes in Escherichia coli was carried out by operon fusion techniques with a hybrid bacteriophage Mu, which creates transcriptional fusions of the structural gene of beta-galactosidase to the host. Among two hundred transductants tested, nine colonies showed higher expression of more than twofold when they were treated with electric pulses. An electric-pulse stimulated transductant was not stimulated by UV irradiation, which is known to induce an SOS response. Conversely, strain PQ37, which has an operon fusion in one of the SOS genes, did not respond to an electric pulse treatment. A possibility of the presence of the electric-pulse stimulated genes, which were not induced by DNA damages, was suggested.
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页码：719 / 722
页数：4
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