THE SIMPLIRED-D DIMER TEST - A NOVEL ASSAY FOR THE DETECTION OF CROSS-LINKED FIBRIN DEGRADATION PRODUCTS IN WHOLE-BLOOD

被引：84

作者：

JOHN, MA ^{[1
]}

ELMS, MJ ^{[1
]}

OREILLY, EJ ^{[1
]}

RYLATT, DB ^{[1
]}

BUNDESEN, PG ^{[1
]}

HILLYARD, CJ ^{[1
]}

机构：

[1] ROYAL BRISBANE HOSP, DEPT HAEMATOL, BRISBANE, QLD 4029, AUSTRALIA

来源：

THROMBOSIS RESEARCH | 1990年 / 58卷 / 03期

关键词：

Bispecific antibody; D dimer; whole blood immunoassay;

D O I：

10.1016/0049-3848(90)90097-V

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

A new system for the detection of fibrin degradation products in whole blood has been developed. The test provides a clearly visible agglutination of the patient's red blood cells in the presence of elevated levels of the crosslinked fibrin derivative, D dimer. The test, which uses a bispecific reagent prepared from Fab' fragments of monoclonal antibodies, gives a positive result in 1-2 minutes. One monoclonal antibody (RAT-1C3/86) was raised against human red blood cells, and the second (DD-3B6/22) was specific to the crosslinked fibrin derivative, D dimer. Addition of the bispecific reagent to a drop of patient's whole blood resulted in red blood cell agglutination when elevated levels of D dimer were present in the sample. Clinical trials showed sensitivity equivalent to that of current commercial tests. Samples from patients with thrombotic disease states as well as normals were examined. The test was compared with commercial latex agglutination and enzyme immunoassay systems and showed good correlation with the presence of elevated levels of crosslinked fibrin degradation products. This technology represents an advance which allows rapid "on the spot" whole blood analysis, for the diagnosis of thrombotic disorders. © 1990.

引用

页码：273 / 281

页数：9

共 14 条

[11]

MERSKEY C, 1969, P SOC EXP BIOL MED, V131, P871

[12] AN IMMUNOASSAY FOR HUMAN D-DIMER USING MONOCLONAL-ANTIBODIES [J].

RYLATT, DB ;

BLAKE, AS ;

COTTIS, LE ;

MASSINGHAM, DA ;

FLETCHER, WA ;

MASCI, PP ;

WHITAKER, AN ;

ELMS, M ;

BUNCE, I ;

WEBBER, AJ ;

WYATT, D ;

BUNDESEN, PG .

THROMBOSIS RESEARCH, 1983, 31 (06) :767-778

[13]

RYLATT DB, 1990, AUST MED J, V152, P75

[14] MEASUREMENT OF CROSS-LINKED FIBRIN DERIVATIVES IN PLASMA - AN IMMUNOASSAY USING MONOCLONAL-ANTIBODIES [J].

WHITAKER, AN ;

ELMS, MJ ;

MASCI, PP ;

BUNDESEN, PG ;

RYLATT, DB ;

WEBBER, AJ ;

BUNCE, IH .

JOURNAL OF CLINICAL PATHOLOGY, 1984, 37 (08) :882-887

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