The Effect Of Different 2,4-Dichlorophenoxyacetic Acid Doses On Chromosomal Structure Of Regenerants In Barley (Hordeum vulgare L.) Embryo Culture

In this study, as a plant material, two-row barley (Hordeum vulgare L.) cultivars called 'Bulbul-89' and 'Tarm-92', which have great importance in Turkey's agricultural production, and as a synthetic auxin 2,4dichlorophenoxyacetic acid (2,4-D) were used. This research, employed 2,4-D as an auxin for callus induction for mature barley embryos in different doses. In both cultivars, the highest values of callus induction, callus weight, regeneration capacity and culture efficiency were established when 3 mg l(-1) 2,4-D auxin was used. In the cytological analysis of the root tips of regenerated plantlets, at 3 mg l(-1) 2,4-D, no physical and numerical abnormality were observed in chromosomes, therefore the doses in question can be used confidently.