STRUCTURES OF GLUCOLIPIDS FROM THE MEMBRANE OF ACHOLEPLASMA-LAIDLAWII STRAIN A-EF22 .1. GLYCEROPHOSPHORYLDIGLUCOSYLDIACYLGLYCEROL AND MONOACYLBISGLYCEROPHOSPHORYLDIGLUCOSYLDIACYLGLYCEROL

The structures of two phosphoglucolipids from the membrane of Acholeplasma laidlnwii, strain A-EF22 were determined by high resolution C-13-, P-31 and H-1-NMR. The lipids in question are 1,2-diacyl-3-O-[glycerophosphoryl-6-O-(alpha-D-glucopyranosyl- (1 --> 2)-O-alpha-D-gloucopyranosyl)]-sn-glycerol (1) and 1,2-diacyl-3-O-[glycerophosphoryl-6-O-(alpha-D-glucopyranosyl-(1 --> 2)-monoacylglycerophosphoryl-6-O-alpha-D-glucopyranosyl)]-sn-glycerol (2). Both lipids are thus derivatives of diglucosyldiacylglycerol. Previous reports on these lipids, based on insufficient chemical analyses, showed contradictory structures. A phosphoglycolipid having the structure of 2 has not been described previously.