3-DIMENSIONAL MAMMALIAN-CELL GROWTH ON NONWOVEN POLYESTER FABRIC DISKS

被引：12
作者：
PETTI, SA [1 ]
LAGES, AC [1 ]
SUSSMAN, MV [1 ]
机构：
[1] TUFTS UNIV,DEPT CHEM ENGN,MEDFORD,MA 02155
来源：
BIOTECHNOLOGY PROGRESS | 1994年 / 10卷 / 05期
关键词：
D O I：
10.1021/bp00029a015
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Small disks of nonwoven polyester fabric (NWPF), similar to Fibra-Cel carriers, were surface treated with strong acid and used as a high surface area support matrix for in vitro culture of anchorage dependent MRC-5 cells. The disks can be autoclaved, and require inocula concentrations of only 2-5% of the final cell densities, which have reached 10(8) cells/mL of disk bed volume. Scanning electron microscopy photographs shown herein re veal that cells grow in a multilayered fashion between the randomly arrayed fibers of the nonwoven fabric, emulating in vivo growth.
引用
收藏
页码：548 / 550
页数：3
相关论文
共 12 条
  • [1] BAKER DJ, 1990, THESIS TUFTS U MEDFO
  • [2] BAKER DJ, 1991, INDA J, V3, P44
  • [3] BOHAK Z, 1989, Patent No. 2178447
  • [4] FELDMAN AF, 1986, THESIS TUFTS U MEDFO
  • [5] KADOURI A, 1987, BIOPOLYMERS, V26, pS205
  • [6] KADOURI A, 1989, MAY INT NONW FABR C, P477
  • [7] USE OF A STATIONARY BED REACTOR AND SERUM-FREE MEDIUM FOR THE PRODUCTION OF RECOMBINANT PROTEINS IN INSECT CELLS
    KOMPIER, R
    KISLEV, N
    SEGAL, I
    KADOURI, A
    [J]. ENZYME AND MICROBIAL TECHNOLOGY, 1991, 13 (10) : 822 - 827
  • [8] Mirchamsy H, 1976, Dev Biol Stand, V37, P297
  • [9] PETTII S, 1993, THESIS TUFTS U MEDFO
  • [10] VERDON SL, 1990, SCANNING MICROSCOPY, V4, P341
12