ACTIONS OF AGONISTS AND CONVULSANT ANTAGONISTS ON A DROSOPHILA-MELANOGASTER GABA RECEPTOR (RD1) HOMO-OLIGOMER EXPRESSED IN XENOPUS OOCYTES

被引：91

作者：

BUCKINGHAM, SD ^{[1
]}

HOSIE, AM ^{[1
]}

ROUSH, RL ^{[1
]}

SATTELLE, DB ^{[1
]}

机构：

[1] UNIV CAMBRIDGE,BABRAHAM INST,DEPT ZOOL,MOLEC SIGNALLING LAB,CAMBRIDGE CB2 3EJ,ENGLAND

来源：

NEUROSCIENCE LETTERS | 1994年 / 181卷 / 1-2期

关键词：

GABA RECEPTOR AGONIST; DROSOPHILA MELANOGASTER; RECEPTOR GENE EXPRESSION; CONVULSANT ANTAGONIST; DIELDRIN RESISTANCE LOCUS;

D O I：

10.1016/0304-3940(94)90578-9

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Cytoplasmic injection of Xenopus oocytes with cDNA-derived RNA (cRNA) encoding a wild-type Drosophila melanogaster GABA receptor subunit (Rdl) resulted in functional expression of a GABA receptor homo-oligomer. Membrane currents recorded in response to muscimol, trans (TACA)- and cis (CACA)-4-aminocrotonic acid reversed at membrane potentials close to E(Cl)- and were insensitive to 1.0 x 10(-4) M bicuculline methiodide. An order of potency of GABA similar to muscimol similar to TACA > CACA > glycine was observed. Response of the expressed Drosophila Rdl receptor to GABA was reduced by t-butylbicyclophosphorothionate (TBPS), 4-n-propyl-4'-ethynylbicycloorthobenzoate (EBOB), picrotoxinin and the novel insecticide, fipronil (all at 1.0 x 10(-5) M).

引用

页码：137 / 140

页数：4

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