PULSED UV LASER-INDUCED FLUORESCENCE DETECTION OF NATIVE PEPTIDES AND PROTEINS IN CAPILLARY ELECTROPHORESIS

被引：54

作者：

CHAN, KC

JANINI, GM

MUSCHIK, GM

ISSAQ, HJ

机构：

[1] Program Resources, Inc./DynCrop, NCI-Frederick Cancer Research and Development Center, Frederick, Maryland 21702, P.O. Box B

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY | 1993年 / 16卷 / 9-10期

关键词：

D O I：

10.1080/10826079308019903

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A pulsed UV laser operating at 248 nm was used for the laser-induced fluorescence (LIF) detection of native tryptophan-containing compounds in capillary electrophoresis. The limit of detection (LOD) of tryptophan was found to be 3.3x10(-9) M (S/N=2). The LODs of the model proteins conalbumin and bovine serum albumin were found to be 1.3x10(-9) and 4x10(-9) M (S/N=2), respectively. These results were at least two orders of magnitude more sensitive when compared to UV absorption at 214 nm.

引用

页码：1877 / 1890

页数：14

共 14 条

[1]

DIPPLE A, 1984, ACS MONOGR SER 182, V1, P41

[2]

KONEV SV, 1967, FLUORESCENCE PHOSPHO, P23

[3]

KUHR WG, 1992, ANAL CHEM, V64, pR389

[4] HIGH-SENSITIVITY LASER-INDUCED FLUORESCENCE DETECTION OF NATIVE PROTEINS IN CAPILLARY ELECTROPHORESIS [J].

LEE, TT ;

YEUNG, ES .

JOURNAL OF CHROMATOGRAPHY, 1992, 595 (1-2) :319-325

[5]

LONGWORTH JW, 1971, EXCITED STATES PROTE, pCH11

[6] STATISTICAL DETERMINATION OF THE AVERAGE VALUES OF THE EXTINCTION COEFFICIENTS OF TRYPTOPHAN AND TYROSINE IN NATIVE PROTEINS [J].

MACH, H ;

MIDDAUGH, CR ;

LEWIS, RV .

ANALYTICAL BIOCHEMISTRY, 1992, 200 (01) :74-80

[7] INDIRECT TIME-RESOLVED LUMINESCENCE DETECTION IN CAPILLARY ZONE ELECTROPHORESIS [J].

NIELEN, MWF .

JOURNAL OF CHROMATOGRAPHY, 1992, 608 (1-2) :85-92

[8] RECENT ADVANCES IN CAPILLARY ELECTROPHORESIS OF PROTEINS, PEPTIDES AND AMINO-ACIDS [J].

NOVOTNY, MV ;

COBB, KA ;

LIU, JP .

ELECTROPHORESIS, 1990, 11 (09) :735-749

[9]

PHILLIPS DH, 1990, CHEM CARCINOGENESIS, V1, P503

[10]

Schmid F.X., 1989, PROTEIN STRUCTURE PR

← 1 2 →