EFFECT OF FREEZING ON THE RELATIVE ESCAPE FORCE OF SPERM AS MEASURED BY A LASER OPTICAL TRAP

被引：23
|
作者：
DANTAS, ZN
ARAUJO, E
TADIR, Y
BERNS, MW
SCHELL, MJ
STONE, SC
机构：
[1] UNIV CALIF IRVINE,DEPT OBSTET & GYNECOL,DIV REPROD ENDOCRINOL & INFERTIL,ORANGE,CA 92613
[2] UNIV CALIF IRVINE,CTR CANC,DEPT MED,DIV HEMATOL ONCOL,ORANGE,CA 92668
[3] BECKMAN LASER INST & MED CLIN,IRVINE,CA
[4] MED CLIN,IRVINE,CA
来源：
FERTILITY AND STERILITY | 1995年 / 63卷 / 01期
关键词：
NORMAL SPERM; FREEZING THAWING; ESCAPE FORCE;
D O I：
10.1016/S0015-0282(16)57316-6
中图分类号：
R71 [妇产科学];
学科分类号：
100211 ;
摘要：
Objective: To determine the possible effect of freezing on sperm escape force as measured by laser trap. Design: Controlled clinical study. Setting: Normal volunteers, academic setting. Patients: Normal, healthy volunteers. Intervention: Sperm selection, freezing and thawing. Measurement of relative (sperm) escape force before and after freezing. Main Outcome Measure: Comparison of escape force as a measurement of freezing effects. Results: Wide individual variations noted, with some individuals showing significant increases or decreases. The relative escape force in fresh (76.1 +/- 31.1 mW) and in frozen-thawed samples (75.6 +/- 40.0 mW) were similar. Conclusion: Freezing does not affect the relative escape force of normal sperm. Any possible detrimental effect of freezing on sperm may be related to other sperm functions.
引用
收藏
页码：185 / 188
页数：4
相关论文
共 27 条
  • [1] EXPOSURE OF HUMAN SPERMATOZOA TO THE CUMULUS-OOPHORUS RESULTS IN INCREASED RELATIVE FORCE AS MEASURED BY A 760 NM LASER OPTICAL TRAP
    WESTPHAL, LM
    ELDANSASOURI, I
    SHIMIZU, S
    TADIR, Y
    BERNS, MW
    HUMAN REPRODUCTION, 1993, 8 (07) : 1083 - 1086
  • [2] MICROMANIPULATION OF SPERM BY A LASER GENERATED OPTICAL TRAP
    TADIR, Y
    WRIGHT, WH
    VAFA, O
    ORD, T
    ASCH, RH
    BERNS, MW
    FERTILITY AND STERILITY, 1989, 52 (05) : 870 - 873
  • [3] FORCE GENERATED BY HUMAN SPERM CORRELATED TO VELOCITY AND DETERMINED USING A LASER GENERATED OPTICAL TRAP
    TADIR, Y
    WRIGHT, WH
    VAFA, O
    ORD, T
    ASCH, RH
    BERNS, MW
    FERTILITY AND STERILITY, 1990, 53 (05) : 944 - 947
  • [4] CONTROLLED MICROMANIPULATION OF HUMAN SPERM IN 3 DIMENSIONS WITH AN INFRARED-LASER OPTICAL TRAP - EFFECT ON SPERM VELOCITY
    COLON, JM
    SAROSI, P
    MCGOVERN, PG
    ASKIN, A
    DZIEDZIC, JM
    SKURNICK, J
    WEISS, G
    BONDER, EM
    FERTILITY AND STERILITY, 1992, 57 (03) : 695 - 698
  • [5] The Effect of Phosphate on the Force Generating Capacity of a Mini-Ensemble of Myosin Measured in a Laser Trap Assay
    Debold, Edward P.
    Woodward, Mike
    Turner, Matthew A.
    BIOPHYSICAL JOURNAL, 2013, 104 (02) : 306A - 306A
  • [6] THE MODIFICACTION OF LASER BEAM FOR OPTIMIZATION OF OPTICAL TRAP FORCE CHARACTERISTICS
    Skidanov, R. V.
    Rykov, M. A.
    COMPUTER OPTICS, 2013, 37 (04) : 431 - 435
  • [7] The Modificaction Of Laser Beam For Optimization Of Optical Trap Force Characteristics
    Skidanov, R. V., 2012, Institution of Russian Academy of Sciences (36)
  • [8] THE MODIFICACTION OF LASER BEAM FOR OPTIMIZATION OF OPTICAL TRAP FORCE CHARACTERISTICS
    Skidanov, R. V.
    Rykov, M. A.
    Iannacchione, G. S.
    Krivoshlykov, S. G.
    COMPUTER OPTICS, 2012, 36 (03) : 377 - 386
  • [9] FORCE GENERATION OF ORGANELLE TRANSPORT MEASURED INVIVO BY AN INFRARED-LASER TRAP
    ASHKIN, A
    SCHUTZE, K
    DZIEDZIC, JM
    EUTENEUER, U
    SCHLIWA, M
    NATURE, 1990, 348 (6299) : 346 - 348
  • [10] Displacement and force from native thick filaments measured with an optical trap.
    Trombetta, A
    Sleep, J
    Simmons, RM
    BIOPHYSICAL JOURNAL, 1997, 72 (02) : TU259 - TU259
123