ORDERED RESTRICTION-ENDONUCLEASE MAPS OF YEAST ARTIFICIAL CHROMOSOMES CREATED BY OPTICAL MAPPING ON SURFACES

被引：94
作者：
CAI, WW
ABURATANI, H
STANTON, VP
HOUSMAN, DE
WANG, YK
SCHWARTZ, DC
机构：
[1] NYU,DEPT CHEM,WM KECK LAB BIOMOLC IMAGING,NEW YORK,NY 10003
[2] MIT,CTR CANC RES,CAMBRIDGE,MA 02139
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1995年 / 92卷 / 11期
关键词：
D O I：
10.1073/pnas.92.11.5164
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
We have developed a surface mounting technology for the rapid construction of ordered restriction maps from individual DNA molecules. Optical restriction maps constructed from yeast artificial chromosome DNA molecules mounted on specially derivatized glass surfaces are accurate and reproducible, and the technology is amenable to automation. The mounting procedures described here should also be useful for fluorescence in situ hybridization studies. We believe these improvements to optical mapping will further stimulate the development of nonelectrophoretic approaches to genome analysis.
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页码：5164 / 5168
页数：5
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