In Vitro Antiproliferative and Antioxidant Activities and Total Phenolic Contents of the Extracts of Melastoma malabathricum Leaves

被引:39
作者
Zakaria, Z. A. [1 ,2 ]
Rofiee, M. S. [3 ]
Mohamed, A. M. [3 ]
Teh, L. K. [2 ]
Salleh, M. Z. [2 ]
机构
[1] Univ Putra Malaysia, Fac Med & Hlth Sci, Dept Biomed Sci, Upm Serdang 43400, Selangor, Malaysia
[2] Univ Teknol MARA, Fac Pharm, Pharmacogen Ctr, Bandar Puncak Alam 42300, Selangor, Malaysia
[3] Univ Ind Selangor, Fac Biotechnol & Life Sci, Shah Alam 40000, Selangor, Malaysia
关键词
antioxidant activity; antiproliferative activity; Melastoma malabathricum; phenolic compounds;
D O I
10.1016/j.jams.2011.09.016
中图分类号
R [医药、卫生];
学科分类号
10 ;
摘要
The present study aims to determine the in vitro antiproliferative and antioxidant activities of various extracts from the leaves of Melastoma malabathricum using various established in vitro assays. The aqueous extract inhibited the proliferation of Caov-3 and HL-60 cell lines, while the chloroform extract exhibited antiproliferative activity against the Caov3, HL-60, and CEM-SS cell lines. The methanol extract demonstrated antiproliferative activity against more cell lines, including the MCF-7, HeLa, Caov-3, HL-60, CEM-SS, and MDA-MB-231 cancer cell lines. Interestingly, all extracts did not inhibit the proliferation of 3T3 cells, thus indicating their noncytotoxic properties. Unlike the chloroform extracts, the aqueous and methanol extracts of M malabathricum (20, 100, and 500 mg/ml) produced high antioxidant activity for the superoxide scavenging assay with only the 500 mg/ml aqueous and methanol extracts exhibited high activity for the 2,2-diphenyl -1-picrylhydrazyl radical scavenging assay. The total phenolic content recorded for the aqueous, methanol, and chloroform extracts were 3344.2 +/- 19.1, 3055.1 +/- 8.7, and 92.5 +/- 7.3 mg/100 g of gallic acid, respectively. The M malabathricum leaves possessed potential antiproliferative and antioxidant activities that could be attributed to its high content of phenolic compounds.
引用
收藏
页码:248 / 256
页数:9
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