GENETIC DIFFERENTIATION OF PHYTOPATHOGENIC FUNGUS COCHLIOBOLUS-SATIVUS (ITO AND KURIB) DRECHSL-EX-DASTUR (BIPOLARIS-SOROKINIANA (SACC, SOROK) SHOEM) REVEALED BY A UNIVERSALLY PRIMED POLYMERASE CHAIN-REACTION UP-PCR TECHNIQUE - CORRELATION WITH HOST-SPECIFICITY

Populations of C. sativus fungus isolated from barley (2 populations, Russia, Kazakhstan) and wheat (3 populations, Kazakhstan, Viet-Nam) have been analysed by UP-PCR technique. The oligoCs2 system consisted of pair universal primers of own construction was used. UP-PCR patterns of isolates of all populations have been divided into 8 UP-PCR polymorphisms. In <<barley>> populations 6 polymorphisms were observed. One from them was represented by a great number of isolates. In <<wheat>> populations there were 4 polymorphisms: the one, represented a majority of isolates, was found in barley also, but in a tew cases, and 2 were unique and wheat-specific. The genetic relationship of isolates represented different PCR polymorphisms was appreciated by the KITSCH (UPGMA-like) and FITCH procedures of PHYLIP package. On the whole the correlation of UP-PCR polymorphisms with the host speciticity of fungus in revealed.