ANALYSIS OF BORDERLINE-RESISTANT STRAINS OF METHICILLIN-RESISTANT STAPHYLOCOCCUS-AUREUS USING POLYMERASE CHAIN-REACTION

被引：69

作者：

HIRAMATSU, K ^{[1
]}

KIHARA, H ^{[1
]}

YOKOTA, T ^{[1
]}

机构：

[1] UNIV TOKYO,FAC MED,DEPT MED & PHYS THERAPY,BUNKYO KU,TOKYO 113,JAPAN

来源：

MICROBIOLOGY AND IMMUNOLOGY | 1992年 / 36卷 / 05期

关键词：

D O I：

10.1111/j.1348-0421.1992.tb02043.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Identification of methicillin-resistant Staphylococcus aureus by drug-susceptibility tests alone poses a serious problem, because a considerable number of clinical S. aureus isolates are borderline resistant to methicillin. To circumvent this problem, we have developed a quick and sensitive method of PCR amplification for the detection of mecA gene, which, coding for PBP2', is the specific genetic element of methicillin-resistant Staphylococcus aureus. This method made it possible to identify MRSA strains in a short time using as few as 30 cells as a starting material for template DNA. Using this method, we found that the strains of borderline methicillin-resistance could be accurately identified. We also found one S. aureus clinical strain, T3, which lacked mecA gene in spite of its resistance to methicillin.

引用

页码：445 / 453

页数：9

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