SIMULTANEOUS HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF ASCORBIC-ACID AND DEHYDROASCORBIC ACID IN BIOLOGICAL SAMPLES

被引：28

作者：

CAMMACK, J

OKE, A

ADAMS, RN

机构：

[1] UNIV KANSAS,SCH PHARM,DEPT PHARMACOL,LAWRENCE,KS 66045

[2] UNIV KANSAS,DEPT CHEM,LAWRENCE,KS 66045

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1991年 / 565卷 / 1-2期

关键词：

D O I：

10.1016/0378-4347(91)80421-8

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The ascorbic acid (AA)-dehydroascorbic acid redox couple is an important component of many biological systems, and various physiological roles have been described for this vitamin. Simultaneous measurement of both AA and dehydroascorbate using high-performance liquid chromatography (HPLC) has proven difficult owing to detection problems. A simple, single-step HPLC assay for the simultaneous detection of both AA and dehydroascorbate was developed without the burden of derivatization of either compounds. This has proven to be a reliable technique and should be applicable to a wide variety of biological samples.

引用

页码：529 / 532

页数：4

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