BOVINE SERUM-ALBUMIN IS A MAJOR OLIGONUCLEOTIDE-BINDING PROTEIN FOUND ON THE SURFACE OF CULTURED-CELLS

被引:49
作者
GESELOWITZ, DA
NECKERS, LM
机构
[1] NIH, CTR CLIN, DEPT NUCL MED, BETHESDA, MD 20892 USA
[2] NCI, CLIN PHARMACOL BRANCH, BETHESDA, MD 20892 USA
来源
ANTISENSE RESEARCH AND DEVELOPMENT | 1995年 / 5卷 / 03期
关键词
D O I
10.1089/ard.1995.5.213
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
To better understand the uptake of oligonucleotides into cells, we have studied the labeling of cell surface proteins by an oligonucleotide conjugated to a radiolabeled photoactivatable crosslinker (Denny-Jaffe reagent). When HL60 cells are treated with the conjugate for 2 hours in a medium containing bovine serum albumin (BSA), almost all of the cell-associated label is found in one protein, which we identify as BSA. Cells grown and treated in a serum-free medium do not show this protein, whereas it is plainly seen in cells that are grown in serum-containing medium but then treated in serum-free medium. Overall association of the oligonucleotide with cells is much higher in serum-free medium than in BSA-containing medium, but the oligonucleotide is mostly not protein-associated in the absence of BSA. We conclude that (1) BSA from the medium serves to block overall association of oligonucleotide with cells, and (2) BSA is the main cell surface protein binding oligonucleotides. We discuss the possible role of albumin in endocytic uptake of oligonucleotides in the cell and in the biodistribution of oligonucleotides in vivo.
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页码:213 / 217
页数:5
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