CURRENT NOISE REVEALS PROTONATION KINETICS AND NUMBER OF IONIZABLE SITES IN AN OPEN PROTEIN ION CHANNEL

被引：167

作者：

BEZRUKOV, SM

KASIANOWICZ, JJ

机构：

[1] NIDDK,LBM,BIOPHYS UNIT,BETHESDA,MD 20892

[2] NATL INST STAND & TECHNOL,DEPT PHYS,DIV BIOTECHNOL,BIOSENSORS GRP,GAITHERSBURG,MD 20899

来源：

PHYSICAL REVIEW LETTERS | 1993年 / 70卷 / 15期

关键词：

D O I：

10.1103/PhysRevLett.70.2352

中图分类号：

O4 [物理学];

学科分类号：

0702 ;

摘要：

In analogy to current fluctuations found in solid state electronic microstructure devices, excess noise generated by the reversible ionization of sites in a transmembrane ionic channel was observed. By analyzing the pH-dependent fluctuations in the current through fully open single channels formed by the alpha-toxin protein, we were able to evaluate the protonation rate constants, the number of sites participating in the protonation process, and the effect of recharging a single site on the channel conductance.

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页码：2352 / 2355

页数：4

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