IMMUNOHISTOCHEMICAL LOCALIZATION OF A METABOTROPIC GLUTAMATE-RECEPTOR, MGLUR5, IN THE RAT-BRAIN

被引：486
作者：
SHIGEMOTO, R
NOMURA, S
OHISHI, H
SUGIHARA, H
NAKANISHI, S
MIZUNO, N
机构：
[1] KYOTO UNIV,FAC MED,INST IMMUNOL,SAKYO KU,KYOTO 606,JAPAN
[2] KYOTO UNIV,COLL MED TECHNOL,SAKYO KU,KYOTO 606,JAPAN
来源：
NEUROSCIENCE LETTERS | 1993年 / 163卷 / 01期
关键词：
METABOTROPIC GLUTAMATE RECEPTOR; MGLUR5; ANTIBODY; IMMUNOHISTOCHEMISTRY; ELECTRON MICROSCOPY; RAT; BRAIN;
D O I：
10.1016/0304-3940(93)90227-C
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
A trpE-fusion protein containing a C-terminal sequence of a rat metabotropic glutamate receptor, mGluR5, was used to produce an antibody. On immunoblot, the antibody specifically reacted with mGluR5 expressed in mammalian cells and rat brain. Immunohistochemical analysis revealed intense mGluR5-like immunoreactivity (LI) in the olfactory bulb, anterior olfactory nuclei, olfactory tubercle, cerebral cortex, hippocampus, lateral septum, striatum, nucleus accumbens, inferior colliculus, and spinal trigeminal nuclei. The distribution pattern of mGluR5-LI corresponds very well with that of mGluR5 mRNA. Electron microscope analysis of the striatum revealed dense accumulation of immunoreaction products in dendrites which were often provided with asymmetrical synapses. These results suggest that mGluR5 is predominantly located in postsynaptic elements.
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页码：53 / 57
页数：5
相关论文
共 19 条
[1]  
ABE T, 1992, J BIOL CHEM, V267, P13361
[2]   INDUCTION OF LTP IN THE HIPPOCAMPUS NEEDS SYNAPTIC ACTIVATION OF GLUTAMATE METABOTROPIC RECEPTORS [J].
BASHIR, ZI ;
BORTOLOTTO, ZA ;
DAVIES, CH ;
BERRETTA, N ;
IRVING, AJ ;
SEAL, AJ ;
HENLEY, JM ;
JANE, DE ;
WATKINS, JC ;
COLLINGRIDGE, GL .
NATURE, 1993, 363 (6427) :347-350
[3]   METABOTROPIC RECEPTORS AND SLOW EXCITATORY ACTIONS OF GLUTAMATE AGONISTS IN THE HIPPOCAMPUS [J].
BASKYS, A .
TRENDS IN NEUROSCIENCES, 1992, 15 (03) :92-96
[4]   CHARACTERIZATION OF LTP INDUCED BY THE ACTIVATION OF GLUTAMATE METABOTROPIC RECEPTORS IN AREA CA1 OF THE HIPPOCAMPUS [J].
BORTOLOTTO, ZA ;
COLLINGRIDGE, GL .
NEUROPHARMACOLOGY, 1993, 32 (01) :1-9
[5]   EXCITATORY EFFECTS OF ACPD RECEPTOR ACTIVATION IN THE HIPPOCAMPUS ARE MEDIATED BY DIRECT EFFECTS ON PYRAMIDAL CELLS AND BLOCKADE OF SYNAPTIC INHIBITION [J].
DESAI, MA ;
CONN, PJ .
JOURNAL OF NEUROPHYSIOLOGY, 1991, 66 (01) :40-52
[6]  
FOTUHI M, 1993, J NEUROSCI, V13, P2001
[7]   EXCITATORY AMINO-ACIDS ACTING ON METABOTROPIC GLUTAMATE RECEPTORS BROADEN THE ACTION-POTENTIAL IN HIPPOCAMPAL-NEURONS [J].
HU, GY ;
STORM, JF .
BRAIN RESEARCH, 1991, 568 (1-2) :339-344
[8]   DEPENDENCE OF LONG-TERM DEPRESSION ON POSTSYNAPTIC METABOTROPIC GLUTAMATE RECEPTORS IN VISUAL-CORTEX [J].
KATO, N .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (08) :3650-3654
[9]   CELLULAR-LOCALIZATION OF A METABOTROPIC GLUTAMATE RECEPTOR IN RAT-BRAIN [J].
MARTIN, LJ ;
BLACKSTONE, CD ;
HUGANIR, RL ;
PRICE, DL .
NEURON, 1992, 9 (02) :259-270
[10]   SEQUENCE AND EXPRESSION OF A METABOTROPIC GLUTAMATE RECEPTOR [J].
MASU, M ;
TANABE, Y ;
TSUCHIDA, K ;
SHIGEMOTO, R ;
NAKANISHI, S .
NATURE, 1991, 349 (6312) :760-765
12