ISOFORMS OF 14-3-3-PROTEIN CAN FORM HOMODIMERS AND HETERODIMERS IN-VIVO AND IN-VITRO - IMPLICATIONS FOR FUNCTION AS ADAPTER PROTEINS

被引：224

作者：

JONES, DH

LEY, S

AITKEN, A

机构：

[1] NATL INST MED RES,DIV PROT STRUCT,LONDON NW7 1AA,ENGLAND

[2] NATL INST MED RES,DIV CELLULAR IMMUNOL,LONDON NW7 1AA,ENGLAND

来源：

FEBS LETTERS | 1995年 / 368卷 / 01期

基金：

英国医学研究理事会;

关键词：

14-3-3; DIMER; SIGNALING; RAF KINASE; MAP KINASE;

D O I：

10.1016/0014-5793(95)00598-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

14-3-3 proteins play a role in many cellular functions: they bind to and regulate several proteins which: are critical for cell proliferation and differentiation. 14-3-3 proteins exist as diners, and in this study we have shown that diverse 14-3-3 proteins can form both homo- and heterodimers in vitro (by cross-linking studies) and in vivo (by coimmunoprecipitation and Western blot analysis); this interaction is mediated solely through the N-terminal domain of the proteins. The composition of 14-3-3 dimers within a cell may play a key part in the role of this family of proteins as modulators or adapters which facilitate the interaction of distinct components of signalling pathways.

引用

页码：55 / 58

页数：4

共 22 条

[1] 14-3-3-ALPHA AND 14-3-3-DELTA ARE THE PHOSPHORYLATED FORMS OF RAF-ACTIVATING 14-3-3-BETA AND 14-3-3-ZETA - IN-VIVO STOICHIOMETRIC PHOSPHORYLATION IN BRAIN AT A SER-PRO-GLU-LYS MOTIF [J].