APPLICATION OF RADIOLUMINOGRAPHY TO OFF-LINE COUNTING OF RADIOACTIVITY IN HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ELUATES

被引：10

作者：

BABA, S ^{[1
]}

TERAZAWA, Y ^{[1
]}

KIMATA, H ^{[1
]}

SHINOHARA, Y ^{[1
]}

AKIRA, K ^{[1
]}

HASEGAWA, H ^{[1
]}

机构：

[1] KOWA CO LTD,TOKYO RES LABS,TOKYO 189,JAPAN

来源：

JOURNAL OF CHROMATOGRAPHY A | 1994年 / 663卷 / 01期

关键词：

D O I：

10.1016/0021-9673(94)80493-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An off-line counting method for the determination of carbon-14 in HPLC eluates was developed using radioluminography (RLG). A succession of aliquots of the eluate were collected in the flat-bottomed wells of a polystyrene microplate, evaporated to dryness and contacted with an imaging plate, and radioactivity was determined with a Bio-image analyser. The limit of detection was 0.35 Bq per injection. The inter-assay relative standard deviation was less than 3% over the range 2-20 Bq. The RLG off-line counting method was utilized to determine [C-14]eicosapentaenoic acid metabolites formed by rat hepatic microsomes. The results were compared with those obtained with an off-line liquid scintillation counting method and an on-line counting method.

引用

收藏

页码：35 / 41

页数：7

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