AVIDIN-BIOTIN COMPLEXATION FOR ENZYME SENSOR APPLICATIONS

被引：26

作者：

ANZAI, J

HOSHI, T

OSA, T

机构：

来源：

TRAC-TRENDS IN ANALYTICAL CHEMISTRY | 1994年 / 13卷 / 05期

关键词：

D O I：

10.1016/0165-9936(94)85040-2

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The use of an avidin-biotin system in the preparation of enzyme sensors is described. The biotin-labelled enzymes can be immobilized on the avidin-modified electrode surface through avidin-biotin complexation. The various techniques for the surface derivatization with biotin and avidin and for the coupling with enzymes are cited. The possibility of constructing a protein architecture on the electrode is also discussed: this is based on the non-covalent interaction of avidin and biotin.

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页码：205 / 210

页数：6

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