SUSCEPTIBILITY TESTING OF DIENTAMOEBA-FRAGILIS ATCC-30948 WITH IODOQUINOL, PAROMOMYCIN, TETRACYCLINE, AND METRONIDAZOLE

Susceptibility testing was performed on Dientamoeba fragilis ATCC 30948 in a dixenic culture with Klebsiella pneumoniae and Bacteroides vulgatus. D. fragilis was cocultured with the bacteria in TYGM-9 medium (ATCC medium 1171). The activities of antiparasitic drugs were assessed by counting viable D. fragilis trophozoites with a hemacytometer by trypan blue exclusion. The minimal amebicidal concentrations of the following four drugs were determined: iodoquinol at 128 mu g/ml, paromomycin at 16 mu g/ml, tetracycline (questionably) at 32 mu g/ml, and metronidazole at 32 mu g/ml.