HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR THE DETERMINATION OF FREE GOSSYPOL IN CHICKEN LIVER

被引：7
作者：
BOTSOGLOU, NA
机构：
[1] Laboratory of Nutrition, School of Veterinary Medicine, Aristotelian University
来源：
JOURNAL OF CHROMATOGRAPHY | 1991年 / 587卷 / 02期
关键词：
D O I：
10.1016/0021-9673(91)85179-J
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A high-performance liquid chromatographic method for the determination of free gossypol in chicken liver at levels down to 0.5 ppm has been developed. Tissue was deproteinized with acetonitrile in presence of ascorbic acid and the filtrate was subjected to hydrolysis with hydrochloric acid. The liberated pure gossypol was partitioned into chloroform and analysed by gradient elution on a 10-mu-m C18 column. The overall recovery was 83.5 +/- 2.6%, with an overall relative standard deviation of 9%.
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页码：333 / 337
页数：5
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