A SERINE PROTEINASE-INHIBITOR LOCUS AT 18Q21.3 CONTAINS A TANDEM DUPLICATION OF THE HUMAN SQUAMOUS-CELL CARCINOMA ANTIGEN GENE

被引：257

作者：

SCHNEIDER, SS ^{[1
]}

SCHICK, C ^{[1
]}

FISH, KE ^{[1
]}

MILLER, E ^{[1
]}

PENA, JC ^{[1
]}

TRETER, SD ^{[1
]}

HUI, SM ^{[1
]}

SILVERMAN, GA ^{[1
]}

机构：

[1] HARVARD UNIV,CHILDRENS HOSP,SCH MED,DEPT PEDIAT,BOSTON,MA 02115

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1995年 / 92卷 / 08期

关键词：

SERPINS; MASPIN; PLASMINOGEN ACTIVATOR INHIBITOR TYPE 2;

D O I：

10.1073/pnas.92.8.3147

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The squamous cell carcinoma antigen (SCCA) is a member of the ovalbumin family of serine proteinase inhibitors (serpins). A neutral form of the protein is found in normal and some malignant squamous cells, whereas an acidic form is detected exclusively in tumor cells and in the circulation of patients with squamous cell tumors. In this report, we describe the cloning of the SCCA gene from normal genomic DNA. Surprisingly, two genes were found, They were tandemly arrayed and flanked by two other closely related serpins, plasminogen activator inhibitor type 2 (PAI2) and maspin at 18q21.3. The genomic structure of the two genes, SCCA1 and SCCA2, was highly conserved. The predicted amino acid sequences were 92% identical and suggested that the neutral form of the protein was encoded by SCCA1 and the acidic form was encoded by SCCA2. Further characterization of the region should determine whether the differential expression of the SCCA genes plays a causal role in development of more aggressive squamous cell carcinomas.

引用

页码：3147 / 3151

页数：5

共 34 条

[1] BASIC LOCAL ALIGNMENT SEARCH TOOL [J].

ALTSCHUL, SF ;

GISH, W ;

MILLER, W ;

MYERS, EW ;

LIPMAN, DJ .

JOURNAL OF MOLECULAR BIOLOGY, 1990, 215 (03) :403-410

[2]

Ausubel F, 2002, SHORT PROTOCOLS MOL

[3] CLONING THE CHROMOSOMAL BREAKPOINT OF T(14-18) HUMAN LYMPHOMAS - CLUSTERING AROUND JH ON CHROMOSOME-14 AND NEAR A TRANSCRIPTIONAL UNIT ON 18 [J].

BAKHSHI, A ;

JENSEN, JP ;

GOLDMAN, P ;

WRIGHT, JJ ;

MCBRIDE, OW ;

EPSTEIN, AL ;

KORSMEYER, SJ .

CELL, 1985, 41 (03) :899-906

[4]

BILLINGSLEY GD, 1993, AM J HUM GENET, V52, P343

[5] CLONING AND MOLECULAR CHARACTERIZATION OF A HUMAN INTRACELLULAR SERINE PROTEINASE-INHIBITOR [J].

COUGHLIN, P ;

SUN, JR ;

CERRUTI, L ;

SALEM, HH ;

BIRD, P .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (20) :9417-9421

[6]

CROMBACH G, 1989, CANCER, V63, P1337, DOI 10.1002/1097-0142(19890401)63:7<1337::AID-CNCR2820630719>3.0.CO

[7]

2-J

[8] NIGMS HUMAN RODENT SOMATIC-CELL HYBRID MAPPING PANEL-1 AND PANEL-2 [J].

DRWINGA, HL ;

TOJI, LH ;

KIM, CH ;

GREENE, AE ;

MULIVOR, RA .

GENOMICS, 1993, 16 (02) :311-314

[9] PREVENTION OF VERTEBRATE NEURONAL DEATH BY THE CRMA GENE [J].

GAGLIARDINI, V ;

FERNANDEZ, PA ;

LEE, RKK ;

DREXLER, HCA ;

ROTELLO, RJ ;

FISHMAN, MC ;

YUAN, J .

SCIENCE, 1994, 263 (5148) :826-828

[10] A GENETIC-LOCUS CLOSELY LINKED TO A PROTEASE INHIBITOR GENE-COMPLEX CONTROLS THE LEVEL OF MULTIPLE RNA TRANSCRIPTS [J].

HILL, RE ;

SHAW, PH ;

BARTH, RK ;

HASTIE, ND .

MOLECULAR AND CELLULAR BIOLOGY, 1985, 5 (08) :2114-2122

← 1 2 3 4 →