EVALUATION OF ANTICOAGULANT ACTIVITY FOR LOW-MOLECULAR-WEIGHT HEPARIN BY CHROMOGENIC SUBSTRATE - MEASUREMENT OF FACTOR XA AND THROMBIN ACTIVITIES

被引:0
作者
ISHIMITSU, S [1 ]
SUGIYAMA, T [1 ]
ITOH, M [1 ]
NATSUGA, T [1 ]
KOMATSU, H [1 ]
OKADA, S [1 ]
机构
[1] SHIMIZU PHARMACEUT CO LTD, DEPT RES, DIV RES & DEV, SHIMIZU, SHIZUOKA 424, JAPAN
来源
YAKUGAKU ZASSHI-JOURNAL OF THE PHARMACEUTICAL SOCIETY OF JAPAN | 1994年 / 114卷 / 08期
关键词
LOW MOLECULAR-WEIGHT HEPARIN; HEPARIN; ANTICOAGULATION; ANTITHROMBIN ACTIVITY; ANTIFACTOR XA ACTIVITY; ANTITHROMBIN III;
D O I
10.1248/yakushi1947.114.8_611
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
We described an amidolytic method for determining the anticoagulant activity of commercially available low molecular-weight heparin (LMWH) with the use of factor Xa (FXa) and thrombin (FIIa), and a chromogenic peptidyl substrate, S-2222 or S-2238. The procedures were based on the photometric determination of the inactivation of FXa and FIIa after incubation with LMWH in the presence of antithrombin III (AT III). At first, the optimal conditions of FXa and FIIa activities with respect to pH, temperature, and the amounts of AT III and LMWH-International Standards (LMWH-IS) were determined. And then, the anticoagulant activities of various LMWHs were determined under the established conditions. In the comparative study with LMWHs, significant differences were found in anti FXa and anti FIIa activities, and their ratios. The anti FIIa activity was reconfirmed to decrease with decreasing molecular weight of LMWHs. On the other hand, the anti Xa activity was, however, less dependent on the molecular weight of LMWHs.
引用
收藏
页码:611 / 617
页数:7
相关论文
共 17 条
[1]  
BARROWCLIFFE TW, 1988, THROMB HAEMOSTASIS, V60, P1
[2]  
DAVIE EW, 1977, PLASMA PROTEINS, V3, P421
[3]  
DIETRICH CP, 1980, MECHANISM SACCHARIDE, P317
[4]  
FARCEED J, 1989, SEMIN THROMB HEMOST, V15, P440
[5]  
FAREED J, 1988, HAEMOSTASIS, V18, P33
[6]  
FAREED J, 1988, HAEMOSTASIS, V18, P3
[7]   METACHROMATIC ACTIVITY OF HEPARIN AND HEPARIN FRAGMENTS [J].
GRANT, AC ;
LINHARDT, RJ ;
FITZGERALD, GL ;
PARK, JJ ;
LANGER, R .
ANALYTICAL BIOCHEMISTRY, 1984, 137 (01) :25-32
[8]  
HANDELAND GF, 1984, THROMB RES, V35, P627
[9]  
HIRSH J, 1987, THROMB DIATH HAEMO, P348
[10]   THE MOLECULAR-WEIGHT DEPENDENCE OF THE RATE-ENHANCING EFFECT OF HEPARIN ON THE INHIBITION OF THROMBIN, FACTOR-XA, FACTOR-IXA, FACTOR-XIA, FACTOR-XIIA AND KALLIKREIN BY ANTI-THROMBIN [J].
HOLMER, E ;
KURACHI, K ;
SODERSTROM, G .
BIOCHEMICAL JOURNAL, 1981, 193 (02) :395-400