Production and partial characterization of multifunctional lipases by Sporobolomyces ruberrimus using soybean meal, rice meal and sugarcane bagasse as substrates

被引：15

作者：

Ferraz, Lenir Rigoli ^{[1
]}

de Oliveira, Daniela dos Santos ^{[1
]}

Silva, Marceli Fernandes ^{[1
]}

Rigo, Elisandra ^{[1
]}

Di Luccio, Marco ^{[2
]}

Oliveira, J. Vladimir ^{[2
]}

de Oliveira, Debora ^{[2
]}

Treichel, Helen ^{[3
,4
]}

机构：

[1] Univ Reg Integrada Alto Uruguai & Missbes, Dept Engn, Campus Erechim, BR-99700000 Erechim, RS, Brazil

[2] Univ Fed Santa Catarina, Dept Engn Quim & Alimentos, BR-88800000 Florianopolis, SC, Brazil

[3] Univ Fed Rio Grande, Escola Quim & Alimentos, Rua Alfredo Huch 475, BR-96201900 Rio Grande, RS, Brazil

[4] Univ Fed Fronteira Sul, Campus Erechim, BR-99700000 Erechim, RS, Brazil

来源：

BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY | 2012年 / 1卷 / 03期

关键词：

Sporobolomyces ruberrimus; Sugarcane bagasse; Soybean bran; Rice bran; Lipases;

D O I：

10.1016/j.bcab.2012.03.008

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Lipases produced by a newly isolated Sporidiobolus ruberrimus strain have potential catalytic ability for esterification reactions. Lipase activities of about 130.1 U/g, 164.2 U/g and 189.5 U/g were obtained using soybean meal, sugarcane bagasse and rice meal as substrates, respectively. The partial characterization of the crude extracts showed that the evaluated lipases presented, in general, more specificity to short chain alcohols and fatty acids. The extracts presented higher relative activities when stored at room temperature compared to the storage at 4 and 10 degrees C. Lipases showed higher activities at memories of pH around neutrality (6.5) and about 40 degrees C. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：243 / 252

页数：10