Construction of Near Isogenic Lines for Pericarp Color and Evaluation on Their Near isogenicity in Rice

被引:9
|
作者
Wang Xiao-guang [1 ]
Ji Zhi-juan [1 ]
Cai Jing [1 ]
Ma Liang-yong [1 ]
Li Xi-ming [1 ]
Yang Chang-deng [1 ]
机构
[1] China Natl Rice Res Inst, State Key Lab Rice Biol, Hangzhou 310006, Zhejiang, Peoples R China
关键词
pericarp color; near isogenic line; agronomic trait; molecular marker; near isogenicity; rice;
D O I
10.1016/S1672-6308(08)60089-X
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
The inheritance of purple pericarp was studied through genetic analysis using F-2, BC1F1 and F-3 from a cross between a purple pericarp rice variety Zixiangnuo and a white pericarp rice variety Chunjiangnuo 2. Seven pairs of near isogenic lines (NILs) for pericarp color of rice were further constructed. Genetic analysis indicated that the purple pericarp was controlled by two complementary genes (Pb and Pp). Agronomic trait analysis and polymorphism analysis using SSR markers on these seven pairs of NILs were used in the evaluation of near-isogenicity. No significant differences in agronomic traits were found except 1000-grain weight between the NILs. The polymorphic SSR markers for the parents were only detected in target segments of the five pairs of NILs different in Pb, which revealed the ideal near isogenicity of them. However, for the two pairs of NILs different in Pp, the polymorphic markers for the two parents were detected as well as in non-targeted segments of chromosomes 11 and 12, respectively.
引用
收藏
页码:261 / 266
页数:6
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