VALIDATION OF A HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC RADIOIMMUNOASSAY METHOD FOR THE DETERMINATION OF LACIDIPINE IN PLASMA

被引：16
|
作者：
PELLEGATTI, M [1 ]
BRAGGIO, S [1 ]
SARTORI, S [1 ]
FRANCESCHETTI, F [1 ]
BOLELLI, GF [1 ]
机构：
[1] UNIV BOLOGNA,CNR,REPROD ENDOCRINE UNIT,I-40126 BOLOGNA,ITALY
来源：
JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1992年 / 573卷 / 01期
关键词：
D O I：
10.1016/0378-4347(92)80481-5
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A sensitive and reproducible method for the determination of lacidipine, a new potent antihypertensive dihydropyridine, is reported. The method involves solid-phase extraction, reversed-phase high-performance liquid chromatography and radioimmunoassay of the collected fraction. The assay provides a limit of detection of 20 pg/ml of plasma, allowing the determination of trough (24 h) plasma concentrations. The method is suitable for pharmacokinetic studies in man.
引用
收藏
页码：105 / 111
页数：7
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