MAMMALIAN BRAIN PLASMA-MEMBRANES - ISOLATION, ENZYMATIC, AND CHEMICAL CHARACTERIZATION

被引：8

作者：

KRISHNAN, KS ^{[1
]}

BALARAM, P ^{[1
]}

机构：

[1] INDIAN INST SCI, MOLEC BIOPHYS UNIT, BANGALORE 560012, INDIA

来源：

EXPERIMENTAL CELL RESEARCH | 1976年 / 101卷 / 02期

关键词：

D O I：

10.1016/0014-4827(76)90381-5

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Two variants of a simplified procedure for the isolation of plasma membrane fractions from monkey and rat brains were described. The preparations showed marked enrichments in the marker enzymes, (Na+-K+) ATPase, acetylcholinesterase, 5''-nucleotidase and adenylate cyclase. Lipid analysis and a protein electrophoretic pattern were presented. An enzymatic check was made to assess for contamination by other cellular organelles. The amino acid composition of brain membrane proteins showed a resemblance to the reported composition of erythrocyte ghost proteins but differ from myelin proteins.

引用

页码：299 / 306

页数：8

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