CHARACTERIZATION OF LIPOPROTEINS CONTAINING A TRUNCATED FORM OF APOLIPOPROTEIN-B, APOLIPOPROTEIN B32

被引:2
作者
MCCORMICK, SPA
DAY, WA
GEORGE, PM
机构
[1] Pathology Department, Christchurch School of Medicine, Christchurch Hospital, Christchurch
来源
BIOCHIMICA ET BIOPHYSICA ACTA-LIPIDS AND LIPID METABOLISM | 1995年 / 1258卷 / 01期
关键词
HYPOBETALIPOPROTEINEMIA; CHOLESTEROL; HEPARIN-BINDING; IMMUNOBLOTTING; MCA-RH7777; CELL;
D O I
10.1016/0005-2760(95)00096-U
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have characterized the lipoproteins isolated from the plasma of a human subject who was heterozygous for a mutation yielding a truncated apolipoprotein B (apo-B32). The apo-B32 lipoproteins were isolated from the plasma high density lipoprotein (HDL) fraction by heparin-Sepharose chromatography. Although the chemical composition of the apo-B32-containing lipoproteins was similar to that of normal HDL, the mean diameter of the apo-B32 lipoproteins was larger than typical apo-AI-containing HDL particles. On agarose gels, the apo-B32 lipoproteins had pre-beta mobility similar to that of normal very low density lipoproteins. Analysis of the purified apo-B32 lipoproteins by SDS-polyacrylamide gel electrophoresis revealed the presence of both apo-AI and apo-E. In order to further analyze the properties of apo-B32, we developed an apo-B32 expression vector and generated stable rat hepatoma cell lines expressing apo-B32. In these cell lines, the apo-B32 protein was secreted in a d > 1.21 g/ml lipoprotein. Oleic acid supplementation of the cell-culture media had no measurable effect on the density distribution of the apo-B32 lipoproteins that were secreted by the cells.
引用
收藏
页码:49 / 56
页数:8
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