EXTRACELLULAR CA2(+)-DEPENDENT AND INDEPENDENT CALCIUM TRANSIENT IN FETAL MYOTUBES

被引：9

作者：

STRUBE, C

BEURG, M

GEORGESCAULD, D

BOURNAUD, R

SHIMAHARA, T

机构：

[1] LAB NEUROBIOL CELLULAIRE & MOLEC,CNRS,F-91198 GIF SUR YVETTE,FRANCE

[2] CTR RECH PAUL PASCAL,CNRS,F-33600 PESSAC,FRANCE

来源：

PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY | 1994年 / 427卷 / 5-6期

关键词：

CALCIUM RELEASE; DIHYDROPYRIDINE RECEPTOR; MYOGENESIS; FURA-2; SKELETAL MUSCLE;

D O I：

10.1007/BF00374269

中图分类号：

Q4 [生理学];

学科分类号：

071003 ;

摘要：

Spatio-temporal changes in the intracellular calcium concentration [Ca2+](i) of dissociated mice myotubes from 14-day and 18-day-old fetuses were studied using digital imaging analysis of the Ca2+ indicator fura-2. Myotubes from 18-day-old fetuses displayed a transient [Ca2+](i) increase upon electrical stimulation either in nominally calcium-free external solution or in Krebs solution containing 100 mu M lanthanum. Thus, at this developmental stage, membrane depolarization appears to increase [Ca2+](i) by stimulating Ca2+ release from the sarcoplasmic reticulum independently of extracellular Ca2+ influx. Similarly, myotubes from 14-day-old fetuses also showed a calcium transient upon electrical stimulation in Krebs solution. However, in 46% of these myotubes the calcium transient was abolished when Ca2+ entry through calcium channels was suppressed.

引用

收藏

页码：517 / 523

页数：7

相关论文

共 23 条

[1] MECHANISM OF RELEASE OF CALCIUM FROM SARCOPLASMIC-RETICULUM OF GUINEA-PIG CARDIAC-CELLS [J].

BEUCKELMANN, DJ ;

WIER, WG .

JOURNAL OF PHYSIOLOGY-LONDON, 1988, 405 :233-255

[2] VOLTAGE SENSORS OF THE FROG SKELETAL-MUSCLE MEMBRANE REQUIRE CALCIUM TO FUNCTION IN EXCITATION CONTRACTION COUPLING [J].

BRUM, G ;

FITTS, R ;

PIZARRO, G ;

RIOS, E .

JOURNAL OF PHYSIOLOGY-LONDON, 1988, 398 :475-505

[3] MESSENGER-RNA FOR CARDIAC CALCIUM-CHANNEL IS EXPRESSED DURING DEVELOPMENT OF SKELETAL-MUSCLE [J].

CHAUDHARI, N ;

BEAM, KG .

DEVELOPMENTAL BIOLOGY, 1993, 155 (02) :507-515

[4] PROGRESSIVE PREDOMINANCE OF SKELETAL VERSUS CARDIAC TYPES OF EXCITATION-CONTRACTION COUPLING DURING INVITRO SKELETAL MYOGENESIS [J].

COGNARD, C ;

RIVETBASTIDE, M ;

CONSTANTIN, B ;

RAYMOND, G .

PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1992, 422 (02) :207-209

[5] INTRACELLULAR CALCIUM TRANSIENTS INDUCED BY DIFFERENT KINDS OF STIMULUS DURING MYOGENESIS OF RAT SKELETAL-MUSCLE CELLS STUDIED BY LASER CYTOFLUOROMETRY WITH INDO-1 [J].

COGNARD, C ;

CONSTANTIN, B ;

RIVETBASTIDE, M ;

RAYMOND, G .

CELL CALCIUM, 1993, 14 (04) :333-348

[6] CALCIUM RELEASE FROM SARCOPLASMIC-RETICULUM [J].

ENDO, M .

PHYSIOLOGICAL REVIEWS, 1977, 57 (01) :71-108

[7] TIME AND CALCIUM DEPENDENCE OF ACTIVATION AND INACTIVATION OF CALCIUM-INDUCED RELEASE OF CALCIUM FROM THE SARCOPLASMIC-RETICULUM OF A SKINNED CANINE CARDIAC PURKINJE-CELL [J].

FABIATO, A .

JOURNAL OF GENERAL PHYSIOLOGY, 1985, 85 (02) :247-289

[8] STRUCTURAL-ANALYSIS OF MUSCLE DEVELOPMENT - TRANSVERSE TUBULES, SARCOPLASMIC-RETICULUM, AND THE TRIAD [J].

FLUCHER, BE .

DEVELOPMENTAL BIOLOGY, 1992, 154 (02) :245-260

[9] SIMULTANEOUS MATURATION OF TRANSVERSE TUBULES AND SARCOPLASMIC-RETICULUM DURING MUSCLE DIFFERENTIATION IN THE MOUSE [J].

FRANZINIARMSTRONG, C .

DEVELOPMENTAL BIOLOGY, 1991, 146 (02) :353-363

[10] FURA-2 IMAGING OF SPONTANEOUS AND ELECTRICALLY INDUCED OSCILLATIONS OF INTRACELLULAR FREE CA2+ IN RAT MYOTUBES [J].

GROUSELLE, M ;

KOENIG, J ;

LASCOMBE, ML ;

CHAPRON, J ;

MELEARD, P ;

GEORGESCAULD, D .

PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1991, 418 (1-2) :40-50