NOVEL FOLD AND PUTATIVE RECEPTOR-BINDING SITE OF GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR

被引：192

作者：

DIEDERICHS, K

BOONE, T

KARPLUS, PA

机构：

[1] CORNELL UNIV,BIOCHEM MOLEC & CELL BIOL LAB,ITHACA,NY 14853

[2] AMGEN INC,THOUSAND OAKS,CA 91320

来源：

SCIENCE | 1991年 / 254卷 / 5039期

关键词：

D O I：

10.1126/science.1837174

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Granulocyte-macrophage colony-stimulating factor (GM-CSF) stimulates the development of and the cytotoxic activity of white blood cells. Recombinant human GM-CSF has proven useful in the treatment of blood disorders. The structure of GM-CSF, which was determined at 2.4 angstrom resolution by x-ray crystallography, has a novel fold combining a two-stranded antiparallel-beta-sheet with an open bundle of four a helices. Residues implicated in receptor recognition, which are distant in the primary sequence, are on adjacent alpha-helices in the folded protein. A working model for the receptor binding site is presented.

引用

页码：1779 / 1782

页数：4

共 32 条

[1] 3-DIMENSIONAL STRUCTURE OF A GENETICALLY ENGINEERED VARIANT OF PORCINE GROWTH-HORMONE
ABDELMEGUID, SS
SHIEH, HS
SMITH, WW
DAYRINGER, HE
VIOLAND, BN
BENTLE, LA
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (18) : 6434 - 6437
[2] ALTMANN SW, 1991, J BIOL CHEM, V266, P5333
[3] STRUCTURAL DESIGN AND MOLECULAR EVOLUTION OF A CYTOKINE RECEPTOR SUPERFAMILY
BAZAN, JF
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (18) : 6934 - 6938
[4] 3-DIMENSIONAL STRUCTURE OF INTERLEUKIN-2
BRANDHUBER, BJ
BOONE, T
KENNEY, WC
MCKAY, DB
[J]. SCIENCE, 1987, 238 (4834) : 1707 - 1709
[5] CRYSTALLOGRAPHIC R-FACTOR REFINEMENT BY MOLECULAR-DYNAMICS
BRUNGER, AT
KURIYAN, J
KARPLUS, M
[J]. SCIENCE, 1987, 235 (4787) : 458 - 460
[6] BURGESS AW, 1987, BLOOD, V69, P43
[7] THE HUMAN HEMATOPOIETIC COLONY-STIMULATING FACTORS
CLARK, SC
KAMEN, R
[J]. SCIENCE, 1987, 236 (4806) : 1229 - 1237
[8] CLARKLEWIS I, 1988, J IMMUNOL, V141, P881
[9] CREIGHTON TE, 1984, PROTEIN STRUCTURES M, P226
[10] LOW-RESOLUTION STRUCTURE OF RECOMBINANT HUMAN GRANULOCYTE MACROPHAGE COLONY STIMULATING FACTOR
DIEDERICHS, K
JACQUES, S
BOONE, T
KARPLUS, PA
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1991, 221 (01) : 55 - 60

← 1 2 3 4 →