INTERACTION OF HSP70 WITH UNFOLDED PROTEINS - EFFECTS OF TEMPERATURE AND NUCLEOTIDES ON THE KINETICS OF BINDING

被引：329
作者：
PALLEROS, DR
WELCH, WJ
FINK, AL
机构：
[1] UNIV CALIF SANTA CRUZ,DEPT CHEM & BIOCHEM,SANTA CRUZ,CA 95064
[2] UNIV CALIF SAN FRANCISCO,DEPT MED,SAN FRANCISCO,CA 94143
[3] UNIV CALIF SAN FRANCISCO,DEPT PHYSIOL,SAN FRANCISCO,CA 94143
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1991年 / 88卷 / 13期
关键词：
HEAT SHOCK PROTEINS; THERMAL STABILITY; NUCLEOTIDE BINDING;
D O I：
10.1073/pnas.88.13.5719
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Circular dichroism and HPLC gel filtration were used to show that cytosolic hsp70 is thermally stable but undergoes a conformational transition (midpoint, 43-degrees-C; 57-degrees-C in the presence of ATP or ADP) leading to oligomerization. hsp70 binds to unfolded, but not to folded, proteins in a temperature-dependent manner; complex formation is significant only at physiologically relevant temperatures. hsp70 binds ADP more tightly than ATP to form a binary complex, which binds to the unfolded protein more rapidly than free hsp70. ADP also inhibits the ATP-induced dissociation of the hsp70-protein complex. A regulatory role for the hsp70-nucleotide binary complexes is proposed.
引用
收藏
页码：5719 / 5723
页数：5
相关论文
共 22 条
  • [1] INTERACTION OF HSP-70 WITH NEWLY SYNTHESIZED PROTEINS - IMPLICATIONS FOR PROTEIN FOLDING AND ASSEMBLY
    BECKMANN, RP
    MIZZEN, LA
    WELCH, WJ
    [J]. SCIENCE, 1990, 248 (4957) : 850 - 854
  • [2] BRIDGER WA, 1983, CELL ATP, P5
  • [3] CHAPPELL TG, 1987, J BIOL CHEM, V262, P746
  • [4] 70K HEAT-SHOCK RELATED PROTEINS STIMULATE PROTEIN TRANSLOCATION INTO MICROSOMES
    CHIRICO, WJ
    WATERS, MG
    BLOBEL, G
    [J]. NATURE, 1988, 332 (6167) : 805 - 810
  • [5] 3-DIMENSIONAL STRUCTURE OF THE ATPASE FRAGMENT OF A 70K HEAT-SHOCK COGNATE PROTEIN
    FLAHERTY, KM
    DELUCAFLAHERTY, C
    MCKAY, DB
    [J]. NATURE, 1990, 346 (6285) : 623 - 628
  • [6] PEPTIDE BINDING AND RELEASE BY PROTEINS IMPLICATED AS CATALYSTS OF PROTEIN ASSEMBLY
    FLYNN, GC
    CHAPPELL, TG
    ROTHMAN, JE
    [J]. SCIENCE, 1989, 245 (4916) : 385 - 390
  • [7] GREENE LE, 1990, J BIOL CHEM, V265, P6682
  • [8] HOMOLOGOUS PLANT AND BACTERIAL PROTEINS CHAPERONE OLIGOMERIC PROTEIN ASSEMBLY
    HEMMINGSEN, SM
    WOOLFORD, C
    VANDERVIES, SM
    TILLY, K
    DENNIS, DT
    GEORGOPOULOS, CP
    HENDRIX, RW
    ELLIS, RJ
    [J]. NATURE, 1988, 333 (6171) : 330 - 334
  • [9] INTERACTIONS OF MISFOLDED INFLUENZA-VIRUS HEMAGGLUTININ WITH BINDING-PROTEIN (BIP)
    HURTLEY, SM
    BOLE, DG
    HOOVERLITTY, H
    HELENIUS, A
    COPELAND, CS
    [J]. JOURNAL OF CELL BIOLOGY, 1989, 108 (06) : 2117 - 2126
  • [10] TRANSFER OF A BETA-TURN STRUCTURE TO A NEW PROTEIN CONTEXT
    HYNES, TR
    KAUTZ, RA
    GOODMAN, MA
    GILL, JF
    FOX, RO
    [J]. NATURE, 1989, 339 (6219) : 73 - 76
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