AN IMPROVED ERGOSTEROL ASSAY TO ESTIMATE FUNGAL BIOMASS IN ECTOMYCORRHIZAS

被引：152
作者：
MARTIN, F
DELARUELLE, C
HILBERT, JL
机构：
[1] Laboratoire de Microbiologie Forestière, Centre de Recherches Forestières de Nancy, Institut National de la Recherche Agronomique, Seicha, 54280, Champenoux
来源：
MYCOLOGICAL RESEARCH | 1990年 / 94卷
关键词：
EUCALYPTUS-GLOBULUS; PISOLITHUS-TINCTORIUS; CHITIN; ECTOMYCORRHIZAL FUNGI; ERGOSTEROL; FUNGAL BIOMASS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY;
D O I：
10.1016/S0953-7562(09)81333-6
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A simplified procedure for estimating fungal ergosterol from small numbers of ectomycorrhizas is described. Efficient extraction of free ergosterol is achieved with ethanol and ergosterol is then quantified by reversed-phase high performance liquid chromatography. This procedure is suitable for estimating ergosterol levels from many fungal isolates. The procedures has been scaled down allowing fungal infection to be measured in a few ectomycorrhizas. The assay is more sensitive, rapid, and convenient than chitin assay in measuring ectomycorrhizal fungus biomass. The development of symbiotic tissues and extent of fungal colonization in Eucalyptus globulus-Pisolithus tinctorius ectomycorrhizas measured by an ergosterol assay was compared to chitin assay. Ergosterol assay was more sensitive in measuring early growth of the fungal partner.
引用
收藏
页码：1059 / 1064
页数：6
相关论文
共 20 条
[1]   GLUTAMATE-DEHYDROGENASES IN ECTOMYCORRHIZAS OF SPRUCE (PICEA-EXCELSA L) AND BEECH (FAGUS-SYLVATICA L) [J].
DELL, B ;
BOTTON, B ;
MARTIN, F ;
LETACON, F .
NEW PHYTOLOGIST, 1989, 111 (04) :683-692
[2]   REGULATION OF GENE-EXPRESSION IN ECTOMYCORRHIZAS .1. PROTEIN-CHANGES AND THE PRESENCE OF ECTOMYCORRHIZA SPECIFIC POLYPEPTIDES IN THE PISOLITHUS-EUCALYPTUS SYMBIOSIS [J].
HILBERT, JL ;
MARTIN, F .
NEW PHYTOLOGIST, 1988, 110 (03) :339-346
[3]  
MALAJCZUK N, 1990, IN PRESS NEW PHYTOLO
[4]   NITROGEN ASSIMILATION IN MYCORRHIZAS .1. PURIFICATION AND PROPERTIES OF THE NICOTINAMIDE ADENINE-DINUCLEOTIDE PHOSPHATE-SPECIFIC GLUTAMATE-DEHYDROGENASE OF THE ECTOMYCORRHIZAL FUNGUS CENOCOCCUM-GRANIFORME [J].
MARTIN, F ;
MSATEF, Y ;
BOTTON, B .
NEW PHYTOLOGIST, 1983, 93 (03) :415-422
[5]   ASSIMILATION OF (NH4+)-N-15 BY BEECH (FAGUS-SYLVATICA L) ECTOMYCORRHIZAS [J].
MARTIN, F ;
STEWART, GR ;
GENETET, I ;
LETACON, F .
NEW PHYTOLOGIST, 1986, 102 (01) :85-94
[6]  
MARTIN F, 1986, PHYSIOL VEG, V24, P209
[7]  
Matcham S. E., 1984, Microbiological methods for environmental biotechnology, P5
[8]  
MATCHAM SE, 1985, APPL MICROBIOL BIOT, V21, P108
[9]   SELECTIVE STAINING OF FUNGAL HYPHAE IN PARASITIC AND SYMBIOTIC PLANT-FUNGUS ASSOCIATIONS [J].
MEYBERG, M .
HISTOCHEMISTRY, 1988, 88 (02) :197-199
[10]  
OSSWALD WF, 1986, Z NATURFORSCH C, V41, P542
12