ORIGIN AND CHARACTERIZATION OF IMMUNOREACTIVE SOMATOSTATIN IN RAT PURE PANCREATIC-JUICE

被引：4

作者：

SARFATI, P ^{[1
]}

MORISSET, J ^{[1
]}

机构：

[1] UNIV SHERBROOKE,FAC SCI,DEPT BIOL,CTR RECH MECANISMES SECRET,SHERBROOKE J1K 2R1,QUEBEC,CANADA

来源：

PANCREAS | 1990年 / 5卷 / 02期

关键词：

Pancreas; Pancreatic juice; Somato-statin characterization; Somatostatin;

D O I：

10.1097/00006676-199003000-00006

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

This study was undertaken to determine the origin and forms of immunoreactive somatostatin (SLI) present in the pancreatic juice of cannu-lated nonanesthetized rats. The systemic origin of somatostatin (SS) was as-sessed from bolus intravenous injections of either I125-Tyr1-SS-14 or synthetic SS-14 under basal and stimulated pancreatic juice secretion, and from mea-surements of radioactivity and SS-14 in the collected juice. Gel filtration of pooled juice samples indicated that there were no forms of SS corresponding to standards of I125-Tyr1-SS-14 or SS-14, but radioactive material eluted in the area of either I125-Tyr or free iodine. Investigation of the presence of SS-14 in the juice by radioimmunoassay (RIA) led to the identification of a 23-kDa SS-like immunoreactive protein that did not correspond to any of the three known forms of SS present in the pancreatic tissue (Pro-SS, SS-28, and SS-14). By boiling the juice before the RIA, this 23-kDa protein was no longer detectable by RIA. These data suggest that this protein may be a “putative” enzyme, degrading the SS tracer in the RIA. This possibility is supported by the obser-vation that boiled juice lost this capacity of interfering with the RIA. In con-clusion, SS in the pancreatic juice, if present, is not from systemic origin and the juice contains an active thermosensitive substance degrading the SS tracer in the RIA. © 1990 Raven Press, Ltd., New York.

引用

页码：158 / 164

页数：7

共 20 条

[11] Poor P.M., Hauser R.E., Exocrine secretion of pancreatic hor-mones, possible mechanisms, Int J Pancreatol, 2, pp. 277-287, (1987)
[12] Korc M., Magun B.E., Recycling of epidermal growth factor in a human pancreatic carcinoma cell line, Proc Natl Acad Sci USA, 82, pp. 6172-6175, (1985)
[13] Izzo R.S., Praissman M., Receptor-mediated internalization and secretion of cholecystokinin into rat pancreatic duct fluid, Am J Physiol, 253, pp. G445-G452, (1987)
[14] Green G.M., Nasset E.S., Effect of diversion of bile pancreatic juice on pancreatic enzyme secretion and intestinal proteolytic enzyme activity in the rat, Am J Dig Dis, 22, pp. 437-443, (1977)
[15] Green C.M., Lyman R.L., Feedback regulation of pancreatic enzyme secretion as a mechanism for trypsin inhibitor induced hypersecretion in rats, Proc Soc Exp Biol Med, 140, pp. 6-12, (1972)
[16] Brazeau P., Benoit R., Somatostatin physiology in pancreas with blood measurements, Excerpta Med, 200, pp. 439-445, (1979)
[17] Sarfati P., Genik P., Morisset J., Caerulein and secretin induced pancreatic growth: A possible control by endogenous pancreatic somatostatin, Regul Pept, 11, pp. 263-273, (1985)
[18] Benoit R., Bohlen R., Brazeau P., Ling N., Guillemin R., Isolation and characterization of rat pancreatic somatostatin, Endocrinology, 107, pp. 2127-2134, (1980)
[19] Tahara Y., Shima K., Hirota H., Ikegami H., Tanaka A., Ku-Mahara Y., Salivary gland glucagon is a fictitious substance due to tracer-degradating activity resistant to protease inhibitors, Biochim Biophys Res Comm, 113, pp. 340-347, (1983)
[20] Baldissera F., Poulsen K., Holst J.J., Mouse salivary glands secrete a glucagon-degradating enzyme, not glucagon, Endocrinology, 117, pp. 84-87, (1985)

← 1 2 →