ORIGIN AND CHARACTERIZATION OF IMMUNOREACTIVE SOMATOSTATIN IN RAT PURE PANCREATIC-JUICE

被引：4

作者：

SARFATI, P ^{[1
]}

MORISSET, J ^{[1
]}

机构：

[1] UNIV SHERBROOKE,FAC SCI,DEPT BIOL,CTR RECH MECANISMES SECRET,SHERBROOKE J1K 2R1,QUEBEC,CANADA

来源：

PANCREAS | 1990年 / 5卷 / 02期

关键词：

Pancreas; Pancreatic juice; Somato-statin characterization; Somatostatin;

D O I：

10.1097/00006676-199003000-00006

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

This study was undertaken to determine the origin and forms of immunoreactive somatostatin (SLI) present in the pancreatic juice of cannu-lated nonanesthetized rats. The systemic origin of somatostatin (SS) was as-sessed from bolus intravenous injections of either I125-Tyr1-SS-14 or synthetic SS-14 under basal and stimulated pancreatic juice secretion, and from mea-surements of radioactivity and SS-14 in the collected juice. Gel filtration of pooled juice samples indicated that there were no forms of SS corresponding to standards of I125-Tyr1-SS-14 or SS-14, but radioactive material eluted in the area of either I125-Tyr or free iodine. Investigation of the presence of SS-14 in the juice by radioimmunoassay (RIA) led to the identification of a 23-kDa SS-like immunoreactive protein that did not correspond to any of the three known forms of SS present in the pancreatic tissue (Pro-SS, SS-28, and SS-14). By boiling the juice before the RIA, this 23-kDa protein was no longer detectable by RIA. These data suggest that this protein may be a “putative” enzyme, degrading the SS tracer in the RIA. This possibility is supported by the obser-vation that boiled juice lost this capacity of interfering with the RIA. In con-clusion, SS in the pancreatic juice, if present, is not from systemic origin and the juice contains an active thermosensitive substance degrading the SS tracer in the RIA. © 1990 Raven Press, Ltd., New York.

引用

页码：158 / 164

页数：7

共 20 条

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