CRYSTALLOGRAPHIC CHARACTERIZATION OF A CU,ZN SUPEROXIDE-DISMUTASE FROM PHOTOBACTERIUM-LEIOGNATHI

Crystals of a copper-zinc Superoxide dismutase from Photobacterium leiognathi, a luminescent marine bacterium that is the species-specific symbiont of the ponyfish, have been obtained from 2-methyl-2,4-pentanediol solutions. The space group was determined using screenless small-angle precession photographs, and was confirmed by analyzing area detector diffraction data with the XENGEN programs for indexing and refinement. The crystals are monoclinic, space group C2 (a = 126.4 A ̊, b = 87.0 A ̊, c = 44.4 A ̊, β = 92.8 Å), and have two 32,000 Mr dimers per asymmetric unit. The crystals diffract to at least 2.7 Å resolution, are resistant to radiation damage, and are suitable for determination of the structure by X-ray diffraction. © 1990.