PROPERTIES OF CELLOBIASE FROM ASPERGILLUS-NIGER

被引：0

作者：

SINGH, A ^{[1
]}

AGRAWAL, AK ^{[1
]}

ABIDI, AB ^{[1
]}

DARMWAL, NS ^{[1
]}

机构：

[1] NARENDRA DEV UNIV AGR & TECHNOL,DEPT BIOCHEM,FAIZABAD 224229,INDIA

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 1990年 / 34卷 / 03期

关键词：

D O I：

暂无

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Cellobiase enzyme was partially purified from the culture filtrate of Aspergillus niger AS-101 and the general and kinetic properties of the enzyme were examined. The enzyme was unstable on storage. However, it was protected by the addition of BSA, glycerol or sodium azide. Addition of glycerol also protected the enzyme from denaturation due to freezing and thawing. Effect of thiol group reagents revealed the presence of -SH groups at the active site of the enzyme. Different modulators such as metal ions and macroionic compounds illustrated varying effects on the purified cellobiase.

引用

页码：356 / 358

页数：3

共 12 条

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