Synthesis of peptides of Carapax Trionycis and their inhibitory effects on TGF-beta 1-induced hepatic stellate cells

被引：7

作者：

Hu, Chunling ^{[1
]}

Peng, Xiaozhi ^{[1
]}

Tang, Yinpin ^{[1
]}

Liu, Yanwen ^{[1
]}

机构：

[1] Hubei Univ Tradit Chinese Med, Sch Pharm, 1 Huangjia River West Rd, Wuhan 430065, Hubei, Peoples R China

来源：

DRUG DISCOVERIES AND THERAPEUTICS | 2013年 / 7卷 / 06期

关键词：

Synthesis of peptides of Carapax Trionycis; hepatic stellate cells; extracellular matrix (ECM); TGF-beta/Smad;

D O I：

10.5582/ddt.2013.v7.6.248

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

We previous identified the anti-fibrotic active ingredients from Carapax Trionycis as two peptides. Here, we synthesized these two peptides (peptide 1 and peptide 2) by a solid phase method and examined their effects on proliferation and activation of cultured hepatic stellate cells (HSC) which are the main ECM (extracellular matrix)-producing cells in fibrosis progression. We demonstrated that peptide 1 and peptide 2 significantly reduced HSC proliferation and activation in a dose dependent manner. Further, peptide 1 and peptide 2 could interfere with TGF-signaling by down-regulating Smad 3 phosphorylation. Thus, these synthetic peptides of Carapax Trionycis could inhibit proliferation and activation of HSC and might be used as a candidate for treatment of liver fibrosis.

引用

页码：248 / 253

页数：6