ENDOGENOUS SULFIDOPEPTIDE LEUKOTRIENE SYNTHESIS AND 12-LIPOXYGENASE ACTIVITY IN BULLFROG (RANA-CATESBEIANA) ERYTHROCYTES

Endogenous leukotriene (LT) synthesis by mammalian inflammatory cells requires both 5-lipoxygenase (5-LO) and 5-lipoxygenase-activating protein. Other myeloid cells, like erythrocytes, have an incomplete 5-lipoxygenase pathway and synthesize leukotrienes transcellularly. Several studies indicate that sulfidopeptide leukotrienes have important physiological functions in bullfrogs and receptors have been characterized. Calcium ionophore activated bullfrog blood was analyzed by reverse phase-high-performance liquid chromatography (RP-HPLC). Endogenous metabolites consisted of 5-LO products including leukotriene D-4. Other metabolites also suggested 12-lipoxygenase activity. Following purification, metabolites from activated erythrocytes were analyzed by RP-HPLC coupled with radioimmunoassay. Erythrocytes demonstrated endogenous synthesis of LTD, which was inhibited by non-selective (NDGA) and specific (MK886) 5-lipoxygenase inhibitors. Experiments with partially purified erythrocyte cytosol further confirmed 5-LO activity and revealed 12-lipoxygenase activity. HPLC analysis of [1-C-14]arachidonic acid labeled metabolites from activated erythrocytes indicates that most of the available substrate is converted to 12-hydroxy-eicosatetraenoic acid (12-HETE). These novel findings indicate that, in contrast to mammals, bullfrog erythrocytes endogenously synthesize LTD, and large quantities of 12-HETE giving them the potential to contribute directly to inflammatory responses. The evolutionary loss of the nucleus in mammalian erythrocytes appears to be associated with the inability to synthesize leukotrienes endogenously.