STABLE PROTOPLAST ISOLATION AND ITS REGENERATION INTO THALLUS OF THE MARINE GREEN-ALGA ULVA-PERTUSA

被引：0
|
作者：
UCHIDA, A [1 ]
YOSHIKAWA, T [1 ]
ISHIDA, Y [1 ]
SAGA, N [1 ]
机构：
[1] TOKAI UNIV,SCH MARINE SCI & TECHNOL,DEPT MARINE SCI,SHIZUOKA 424,JAPAN
来源：
NIPPON SUISAN GAKKAISHI | 1992年 / 58卷 / 01期
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D O I：
暂无
中图分类号：
S9 [水产、渔业];
学科分类号：
0908 ;
摘要：
Protoplasts were isolated enzymatically from female gametophytes of the marine green alga Ulva pertusa. The best enzyme compositions to yield protoplasts were 5% Abalone Acetone Powder, 2 % Cellulase Onozuka R-10, and 0.1 % Macerozyme R-200 in 1.2 M sorbitol at pH 5.5. The isolated protoplasts regenerated in PES medium and two types of developmental process were observed; thallus forming type and rhizoid and thallus forming type.
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页码：153 / 157
页数：5
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