STABLE PROTOPLAST ISOLATION AND ITS REGENERATION INTO THALLUS OF THE MARINE GREEN-ALGA ULVA-PERTUSA

被引：0

作者：

UCHIDA, A ^{[1
]}

YOSHIKAWA, T ^{[1
]}

ISHIDA, Y ^{[1
]}

SAGA, N ^{[1
]}

机构：

[1] TOKAI UNIV,SCH MARINE SCI & TECHNOL,DEPT MARINE SCI,SHIZUOKA 424,JAPAN

来源：

NIPPON SUISAN GAKKAISHI | 1992年 / 58卷 / 01期

关键词：

D O I：

暂无

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

Protoplasts were isolated enzymatically from female gametophytes of the marine green alga Ulva pertusa. The best enzyme compositions to yield protoplasts were 5% Abalone Acetone Powder, 2 % Cellulase Onozuka R-10, and 0.1 % Macerozyme R-200 in 1.2 M sorbitol at pH 5.5. The isolated protoplasts regenerated in PES medium and two types of developmental process were observed; thallus forming type and rhizoid and thallus forming type.

引用

页码：153 / 157

页数：5

共 15 条

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