A COMPARISON OF AMIDE AND UREIDE NITROGEN-SOURCES IN TISSUE-CULTURE OF TREE LEGUME PROSOPIS-ALBA CLONE B2 V-50

被引：8

作者：

GREEN, B ^{[1
]}

TABONE, T ^{[1
]}

FELKER, P ^{[1
]}

机构：

[1] TEXAS A&I UNIV,COLL AGR,CAESAR KLEBERG WILDLIFE RES INST,CTR SEMIARID FOREST RESOURCES,KINGSVILLE,TX 78363

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 1990年 / 21卷 / 01期

关键词：

amide; in vitro culture; mesquite; Prosopis alba; shoot development; ureide;

D O I：

10.1007/BF00034497

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The effects of medium nitrogen sources on the recalcitrant nature of Prosopis alba clone B2V50 in tissue culture were compared involving shoot development using axillary bud explants from 2 to 4-year-old greenhouse-grown trees. A significant difference (P<0.05) was found between the amino acids aspartic acid and glutamic acid and their corresponding amide-containing compounds asparagine and glutamine. A comparison between amide and ureide nitrogen sources showed that allantoin, a ureide, was an acceptable replacement for asparagine or glutamine. Allantoin, asparagine, and glutamine could be used as the sole nitrogen sources. Allantoin at a concentration of 20 mM was adopted for use in future research. Although shoots were consistently induced, all explants showed complete shoot-tip necrosis after 12 weeks of in vitro culture. © 1990 Kluwer Academic Publishers.

引用

页码：83 / 86

页数：4

共 18 条

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[2]

Durzan D. J., 1985, Tissue culture in forestry and agriculture. Proceedings of the third Tennessee symposium on plant cell and tissue culture held Sept. 9-13, 1984 at the University of Tennessee, Knoxville., P233

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