MUTANTS OF GAL4 PROTEIN ALTERED IN AN ACTIVATION FUNCTION

被引：247
作者：
GILL, G
PTASHNE, M
机构：
[1] Department of Biochemistry, Molecular Biology Harvard University Cambridge
来源：
CELL | 1987年 / 51卷 / 01期
关键词：
D O I：
10.1016/0092-8674(87)90016-X
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Activating region I of GAL4 has been defined as a region 48 amino acids long which, when attached to GAL4's DNA-binding domain, activates transcription in yeast. Here we describe mutants bearing changes in and around this highly acidic activating region. We find mutations that increase the activation function invariably increase the acidity of the region and some but not all of the mutations that decrease the activation function decrease the acidity of the region. © 1987.
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页码：121 / 126
页数：6
相关论文
共 28 条
[1]  
BRAM R, 1985, EMBO J, V5, P603
[2]   A EUKARYOTIC TRANSCRIPTIONAL ACTIVATOR BEARING THE DNA SPECIFICITY OF A PROKARYOTIC REPRESSOR [J].
BRENT, R ;
PTASHNE, M .
CELL, 1985, 43 (03) :729-736
[3]   ACTIVATION OF TRANSCRIPTION BY THE BACTERIOPHAGE-434 REPRESSOR [J].
BUSHMAN, FD ;
PTASHNE, M .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (24) :9353-9357
[4]   MUTATIONAL SPECIFICITY OF A CONDITIONAL ESCHERICHIA-COLI MUTATOR, MUTD5 [J].
FOWLER, RG ;
DEGNEN, GE ;
COX, EC .
MOLECULAR & GENERAL GENETICS, 1974, 133 (03) :179-191
[5]   SPECIFIC DNA-BINDING OF GAL4, A POSITIVE REGULATORY PROTEIN OF YEAST [J].
GINIGER, E ;
VARNUM, SM ;
PTASHNE, M .
CELL, 1985, 40 (04) :767-774
[6]   MUTANT LAMBDA-PHAGE REPRESSOR WITH A SPECIFIC DEFECT IN ITS POSITIVE CONTROL FUNCTION [J].
GUARENTE, L ;
NYE, JS ;
HOCHSCHILD, A ;
PTASHNE, M .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1982, 79 (07) :2236-2239
[7]   A GAL10-CYC1 HYBRID YEAST PROMOTER IDENTIFIES THE GAL4 REGULATORY REGION AS AN UPSTREAM SITE [J].
GUARENTE, L ;
YOCUM, RR ;
GIFFORD, P .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1982, 79 (23) :7410-7414
[8]   REGULATION OF EXPRESSION OF THE GALACTOSE GENE-CLUSTER IN SACCHAROMYCES-CEREVISIAE - ISOLATION AND CHARACTERIZATION OF THE REGULATORY GENE GAL4 [J].
HASHIMOTO, H ;
KIKUCHI, Y ;
NOGI, Y ;
FUKASAWA, T .
MOLECULAR & GENERAL GENETICS, 1983, 191 (01) :31-38
[9]   REPRESSOR STRUCTURE AND THE MECHANISM OF POSITIVE CONTROL [J].
HOCHSCHILD, A ;
IRWIN, N ;
PTASHNE, M .
CELL, 1983, 32 (02) :319-325
[10]   FUNCTIONAL DISSECTION OF A EUKARYOTIC TRANSCRIPTIONAL ACTIVATOR PROTEIN, GCN4 OF YEAST [J].
HOPE, IA ;
STRUHL, K .
CELL, 1986, 46 (06) :885-894
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