REGULATION OF NA+,K+-ATPASE GENE-EXPRESSION BY INSULIN IN VASCULAR SMOOTH-MUSCLE CELLS

被引：63
作者：
TIRUPATTUR, PR [1 ]
RAM, JL [1 ]
STANDLEY, PR [1 ]
SOWERS, JR [1 ]
机构：
[1] WAYNE STATE UNIV,DEPT MED,DIV ENDOCRINOL & HYPERTENS,DETROIT,MI 48201
来源：
AMERICAN JOURNAL OF HYPERTENSION | 1993年 / 6卷 / 07期
关键词：
NA+; K+-ATPASE; INSULIN; MESSENGER RNA; VASCULAR SMOOTH MUSCLE; SOUTHERN BLOT; POLYMERASE CHAIN REACTION; OUABAIN;
D O I：
10.1093/ajh/6.7.626
中图分类号：
R6 [外科学];
学科分类号：
1002 ; 100210 ;
摘要：
Na+,K+-ATPase gene expression and activity and its modulation by insulin were investigated in vascular smooth muscle cells (VSMC). Messenger RNAs encoding for alpha1- and alpha2-isoforms of Na+,K+-ATPase were identified in a pure population of cultured VSMC (a7r5 cells). The predominant isoform in VSMC was alpha2, but only alpha2 was regulated by insulin. Insulin treatment of VSMC for 1 and 6 h caused significant increases in mRNA encoding the alpha2-subunit of Na+,K+-ATPase (n = 7, P < .01). Under similar conditions, insulin had no effect on alpha1-Na+,K+-ATPase mRNA levels. VSMC treated with insulin exhibited a significant stimulation of ouabain inhibitable ATPase activity (1 h insulin, n = 3, v control, n = 3, P < .025; 6 h insulin, n = 3, v control, n = 3, P < .05). Changes in insulin regulation of Na+,K+-ATPase gene expression in vascular smooth muscle may be an important factor in the development of hypertension in diabetes.
引用
收藏
页码：626 / 629
页数：4
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