CONSEQUENCES OF THE OVEREXPRESSION OF UBIQUITIN IN YEAST - ELEVATED TOLERANCES OF OSMOSTRESS, ETHANOL AND CANAVANINE, YET REDUCED TOLERANCES OF CADMIUM, ARSENITE AND PAROMOMYCIN

被引：32

作者：

CHEN, YP ^{[1
]}

PIPER, PW ^{[1
]}

机构：

[1] UNIV LONDON UNIV COLL,DEPT BIOCHEM & MOLEC BIOL,LONDON WC1E 6BT,ENGLAND

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH | 1995年 / 1268卷 / 01期

关键词：

UBIQUITIN; STRESS TOLERANCE; PROTEIN DAMAGE; CADMIUM TOXICITY; (SACCHAROMYCES-CEREVISIAE);

D O I：

10.1016/0167-4889(95)00044-S

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Ubiquitin is induced by diverse stresses in all eukaroytes probably in reflection of the need for more extensive protein turnover by the ubiquitination system in stressed cells. To determine if ubiquitin overexpression can confer general protection against different stresses, yeast cells were engineered to overexpress ubiquitin and the effects of this overexpression on different stress tolerances determined. Ethanol and osmostress tolerances were slightly increased by ubiquitin overexpression, tolerance to heat was unaffected, while still other tolerances were reduced as compared to cells with normal ubiquitin levels. It is noteworthy that tolerance of the amino acid analogue canavanine was markedly increased by ubiquitin overexpression, yet resistance to at least three other agents that contribute to accumulation of aberrant proteins (arsenite, cadmium, paromomycin) was decreased.

引用

页码：59 / 64

页数：6

共 29 条

[1]

CHEN Y, 1994, THESIS U LONDON

[2] OVEREXPRESSION OF THE GENE FOR POLYUBIQUITIN IN YEAST CONFERS INCREASED SECRETION OF A HUMAN-LEUKOCYTE PROTEASE INHIBITOR [J].

CHEN, YP ;

PIOLI, D ;

PIPER, PW .

BIO-TECHNOLOGY, 1994, 12 (08) :819-823

[3] POLYUBIQUITIN GENE-EXPRESSION CONTRIBUTES TO OXIDATIVE STRESS RESISTANCE IN RESPIRATORY YEAST (SACCHAROMYCES-CEREVISIAE) [J].

CHENG, L ;

WATT, R ;

PIPER, PW .

MOLECULAR AND GENERAL GENETICS, 1994, 243 (03) :358-362

[4] UBIQUITIN DEPENDENCE OF SELECTIVE PROTEIN-DEGRADATION DEMONSTRATED IN THE MAMMALIAN-CELL CYCLE MUTANT TS85 [J].

CIECHANOVER, A ;

FINLEY, D ;

VARSHAVSKY, A .

CELL, 1984, 37 (01) :57-66

[5] THE ROLE OF GLUTATHIONE BIOSYNTHESIS IN HEAVY-METAL RESISTANCE IN THE FISSION YEAST SCHIZOSACCHAROMYCES-POMBE [J].

COBLENZ, A ;

WOLF, K .

FEMS MICROBIOLOGY REVIEWS, 1994, 14 (04) :303-308

[6] THE YEAST POLYUBIQUITIN GENE IS ESSENTIAL FOR RESISTANCE TO HIGH-TEMPERATURES, STARVATION, AND OTHER STRESSES [J].

FINLEY, D ;

OZKAYNAK, E ;

VARSHAVSKY, A .

CELL, 1987, 48 (06) :1035-1046

[7] THERMOLABILITY OF UBIQUITIN-ACTIVATING ENZYME FROM THE MAMMALIAN-CELL CYCLE MUTANT TS85 [J].

FINLEY, D ;

CIECHANOVER, A ;

VARSHAVSKY, A .

CELL, 1984, 37 (01) :43-55

[8] THE TAILS OF UBIQUITIN PRECURSORS ARE RIBOSOMAL-PROTEINS WHOSE FUSION TO UBIQUITIN FACILITATES RIBOSOME BIOGENESIS [J].

FINLEY, D ;

BARTEL, B ;

VARSHAVSKY, A .

NATURE, 1989, 338 (6214) :394-401

[9] UBIQUITINATION [J].

FINLEY, D ;

CHAU, V .

ANNUAL REVIEW OF CELL BIOLOGY, 1991, 7 :25-69

[10] UBIQUITIN GENE-EXPRESSION - RESPONSE TO ENVIRONMENTAL-CHANGES [J].

FRASER, J ;

LUU, HA ;

NECULCEA, J ;

THOMAS, DY ;

STORMS, RK .

CURRENT GENETICS, 1991, 20 (1-2) :17-23

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